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Gudagudi KB, d'Entrèves NP, Woudberg NJ, et al. In vitro induction of quiescence in isolated primary human myoblasts. Cytotechnology. 2020;72(2):189-202doi: 10.1007/s10616-019-00365-8.
Gudagudi, K. B., d'Entrèves, N. P., Woudberg, N. J., Steyn, P. J., & Myburgh, K. H. (2020). In vitro induction of quiescence in isolated primary human myoblasts. Cytotechnology, 72(2), 189-202. https://doi.org/10.1007/s10616-019-00365-8
Gudagudi, Kirankumar B, et al. "In vitro induction of quiescence in isolated primary human myoblasts." Cytotechnology vol. 72,2 (2020): 189-202. doi: https://doi.org/10.1007/s10616-019-00365-8
Gudagudi KB, d'Entrèves NP, Woudberg NJ, Steyn PJ, Myburgh KH. In vitro induction of quiescence in isolated primary human myoblasts. Cytotechnology. 2020 Apr;72(2):189-202. doi: 10.1007/s10616-019-00365-8. Epub 2020 Jan 28. PMID: 31993891; PMCID: PMC7192999.
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Cytotechnology. 2020 Apr;72(2):189-202. doi: 10.1007/s10616-019-00365-8. Epub 2020 Jan 28.

In vitro induction of quiescence in isolated primary human myoblasts.

Cytotechnology

Kirankumar B Gudagudi, Niccolò Passerin d'Entrèves, Nicholas J Woudberg, Paul J Steyn, Kathryn H Myburgh

Affiliations

  1. Department of Physiological Sciences, Stellenbosch University, Matieland, Private Bag X1, Stellenbosch, 7602, South Africa.
  2. Department of Human Biology, University of Cape Town, Anzio Road, Observatory, South Africa.
  3. Department of Physiological Sciences, Stellenbosch University, Matieland, Private Bag X1, Stellenbosch, 7602, South Africa. [email protected].

PMID: 31993891 PMCID: PMC7192999 DOI: 10.1007/s10616-019-00365-8

Abstract

Adult skeletal muscle stem cells, satellite cells, remain in an inactive or quiescent state in vivo under physiological conditions. Progression through the cell cycle, including activation of quiescent cells, is a tightly regulated process. Studies employing in vitro culture of satellite cells, primary human myoblasts (PHMs), necessitate isolation myoblasts from muscle biopsies. Further studies utilizing these cells should endeavour to represent their native in vivo characteristics as closely as possible, also considering variability between individual donors. This study demonstrates the approach of utilizing KnockOut™ Serum Replacement (KOSR)-supplemented culture media as a quiescence-induction media for 10 days in PHMs isolated and expanded from three different donors. Cell cycle analysis demonstrated that treatment resulted in an increase in G

Keywords: Cell cycle inhibition; Isolation; Primary human myoblasts; Quiescence

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