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Wan YM, Wu HM, Li YH, et al. TSG-6 Inhibits Oxidative Stress and Induces M2 Polarization of Hepatic Macrophages in Mice With Alcoholic Hepatitis . Front Pharmacol. 2020;11:10doi: 10.3389/fphar.2020.00010.
Wan, Y. M., Wu, H. M., Li, Y. H., Xu, Z. Y., Yang, J. H., Liu, C., He, Y. F., Wang, M. J., Wu, X. N., & Zhang, Y. (2020). TSG-6 Inhibits Oxidative Stress and Induces M2 Polarization of Hepatic Macrophages in Mice With Alcoholic Hepatitis . Frontiers in pharmacology, 1110. https://doi.org/10.3389/fphar.2020.00010
Wan, Yue-Meng, et al. "TSG-6 Inhibits Oxidative Stress and Induces M2 Polarization of Hepatic Macrophages in Mice With Alcoholic Hepatitis ." Frontiers in pharmacology vol. 11 (2020): 10. doi: https://doi.org/10.3389/fphar.2020.00010
Wan YM, Wu HM, Li YH, Xu ZY, Yang JH, Liu C, He YF, Wang MJ, Wu XN, Zhang Y. TSG-6 Inhibits Oxidative Stress and Induces M2 Polarization of Hepatic Macrophages in Mice With Alcoholic Hepatitis . Front Pharmacol. 2020 Feb 04;11:10. doi: 10.3389/fphar.2020.00010. eCollection 2020. PMID: 32116692; PMCID: PMC7010862.
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Front Pharmacol. 2020 Feb 04;11:10. doi: 10.3389/fphar.2020.00010. eCollection 2020.

TSG-6 Inhibits Oxidative Stress and Induces M2 Polarization of Hepatic Macrophages in Mice With Alcoholic Hepatitis .

Frontiers in pharmacology

Yue-Meng Wan, Hua-Mei Wu, Yu-Hua Li, Zhi-Yuan Xu, Jin-Hui Yang, Chang Liu, Yue-Feng He, Men-Jie Wang, Xi-Nan Wu, Yuan Zhang

Affiliations

  1. Gastroenterology Department, the 2nd Affiliated Hospital of Kunming Medical University, Kunming, China.
  2. Department of Occupational, Labor and Environmental Health, Public Health Institute of Kunming Medical University, Kunming, China.
  3. The Biomedical Engineering Research Center, Kunming Medical University, Kunming, China.

PMID: 32116692 PMCID: PMC7010862 DOI: 10.3389/fphar.2020.00010

Abstract

Tumor necrosis factor (TNF)-α-stimulated protein 6 (TSG-6) is a secreted protein with diverse tissue protective and anti-inflammatory properties. We aimed to investigate its effective in treating mice with alcoholic hepatitis (AH) and the associated mechanisms. AH was induced in 8-10 week female C57BL/6N mice by chronic-binge ethanol feeding for 10 days. Intraperitoneal (i.p.) injection of recombinant mouse TSG-6 or saline were performed in mice on day 10. Blood samples and hepatic tissues were collected on day 11. Biochemistry, liver histology, flow cytometry, and cytokine measurements were conducted. Compared to the normal control mice, the AH mice had significantly increased liver/body weight ratio, serum alanine aminotransferase (ALT) and aspartate aminotransferases (AST), hepatic total cholesterol (TC), triglyceride (TG), malondialdehyde (MDA), hepatic macrophage infiltration, serum and hepatic interleukin (IL)-6, and tumor necrosis factor (TNF)-α, which were markedly reduced by i.p. injection of rmTSG-6. Compared to the normal control mice, the hepatic glutathione (GSH), accumulation of M2 macrophages, serum, and hepatic IL-10 and TSG-6 were prominently reduced in the AH mice, which were significantly enhanced after i.p. injection of rmTSG-6. Compared to the normal control mice, hepatic activation of signal transducer and activator of transcription 3 (STAT3) was significantly induced, which was markedly suppressed by rmTSG-6 treatment. TSG-6 were effective for the treatment of AH mice, which might be associated with its ability in inhibiting hepatic oxidative stress and inducing hepatic M2 macrophages polarization

Copyright © 2020 Wan, Wu, Li, Xu, Yang, Liu, He, Wang, Wu and Zhang.

Keywords: STAT3 activation; TSG-6; alcoholic hepatitis; macrophage polarization; oxidative stress

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