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Liao H, Huang T, Hu L, et al. Fluorescent aptasensors for parallel analysis of biomolecules based on interlocked DNA catenane nanomachines. Anal Chim Acta. 2020;1114:1-6doi: 10.1016/j.aca.2020.04.004.
Liao, H., Huang, T., Hu, L., & Wang, M. (2020). Fluorescent aptasensors for parallel analysis of biomolecules based on interlocked DNA catenane nanomachines. Analytica chimica acta, 11141-6. https://doi.org/10.1016/j.aca.2020.04.004
Liao, Hong, et al. "Fluorescent aptasensors for parallel analysis of biomolecules based on interlocked DNA catenane nanomachines." Analytica chimica acta vol. 1114 (2020): 1-6. doi: https://doi.org/10.1016/j.aca.2020.04.004
Liao H, Huang T, Hu L, Wang M. Fluorescent aptasensors for parallel analysis of biomolecules based on interlocked DNA catenane nanomachines. Anal Chim Acta. 2020 Jun 01;1114:1-6. doi: 10.1016/j.aca.2020.04.004. Epub 2020 Apr 06. PMID: 32359510.
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Anal Chim Acta. 2020 Jun 01;1114:1-6. doi: 10.1016/j.aca.2020.04.004. Epub 2020 Apr 06.

Fluorescent aptasensors for parallel analysis of biomolecules based on interlocked DNA catenane nanomachines.

Analytica chimica acta

Hong Liao, Ting Huang, Lianzhe Hu, Min Wang

Affiliations

  1. Chongqing Key Laboratory of Green Synthesis and Applications, College of Chemistry, Chongqing Normal University, Chongqing, 401331, China.
  2. School of Pharmaceutical Sciences, Chongqing University, Chongqing, 401331, China.
  3. Chongqing Key Laboratory of Green Synthesis and Applications, College of Chemistry, Chongqing Normal University, Chongqing, 401331, China. Electronic address: [email protected].
  4. School of Pharmaceutical Sciences, Chongqing University, Chongqing, 401331, China. Electronic address: [email protected].

PMID: 32359510 DOI: 10.1016/j.aca.2020.04.004

Abstract

Stimuli-responsive DNA catenane nanomachines have received considerable interest in the area of DNA nanotechnology. However, the sensing and bio-sensing applications of DNA catenane nanomachines are rarely explored. Herein, the biological small molecule and protein responsive DNA catenane nanomachines were designed by utilizing the specific aptamer/target interaction. In the presence of the target, the blocked catalytically inactive DNA catenane can be activated, while generating the metal ion-dependent DNAzyme as the signal output unit. The method allows the fluorescent detection of ATP and lysozyme with a detection limit of 20 nM and 200 pM, respectively. More importantly, through the use of different fluorophores as labels, the parallel analysis of ATP and lysozyme in the mixed solution was demonstrated based on two interlocked DNA catenanes. Compared with linear scaffold, the interlocked DNA rings showed enhanced stability against enzyme degradation and easily realized intramolecular reconfiguration. Due to its inherent advantages, the interlocked DNA catenane holds great promise in the field of DNA based sensors and nanodevices.

Copyright © 2020 Elsevier B.V. All rights reserved.

Keywords: Aptamer; Catenane; DNA; DNAzyme; Fluorescence; Nanomachine

Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this pa

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