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Urabe G, Sato T, Nakamura G, et al. 1.2 MV/cm pulsed electric fields promote transthyretin aggregate degradation. Sci Rep. 2020;10(1):12003doi: 10.1038/s41598-020-68681-0.
Urabe, G., Sato, T., Nakamura, G., Kobashigawa, Y., Morioka, H., & Katsuki, S. (2020). 1.2 MV/cm pulsed electric fields promote transthyretin aggregate degradation. Scientific reports, 10(1), 12003. https://doi.org/10.1038/s41598-020-68681-0
Urabe, Gen, et al. "1.2 MV/cm pulsed electric fields promote transthyretin aggregate degradation." Scientific reports vol. 10,1 (2020): 12003. doi: https://doi.org/10.1038/s41598-020-68681-0
Urabe G, Sato T, Nakamura G, Kobashigawa Y, Morioka H, Katsuki S. 1.2 MV/cm pulsed electric fields promote transthyretin aggregate degradation. Sci Rep. 2020 Jul 20;10(1):12003. doi: 10.1038/s41598-020-68681-0. PMID: 32686729; PMCID: PMC7371718.
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Sci Rep. 2020 Jul 20;10(1):12003. doi: 10.1038/s41598-020-68681-0.

1.2 MV/cm pulsed electric fields promote transthyretin aggregate degradation.

Scientific reports

Gen Urabe, Takashi Sato, Gomaru Nakamura, Yoshihiro Kobashigawa, Hiroshi Morioka, Sunao Katsuki

Affiliations

  1. Graduate School of Science and Technology, Kumamoto University, Kumamoto, 860-8555, Japan. [email protected].
  2. Department of Analytical and Biophysical Chemistry, Kumamoto University, Kumamoto, 862-0973, Japan.
  3. Graduate School of Science and Technology, Kumamoto University, Kumamoto, 860-8555, Japan.
  4. Institute of Pulsed Power Science, Kumamoto University, Kumamoto, 860-8555, Japan. [email protected].

PMID: 32686729 PMCID: PMC7371718 DOI: 10.1038/s41598-020-68681-0

Abstract

Numerous theoretical studies have been conducted on the effects of high-voltage electric fields on proteins, but few have produced experimental evidence. To acquire experimental data for the amyloid disassemble theory, we exposed transthyretin aggregates to 1,000 ns 1.26 MV/cm pulsed electric fields (PEFs) to promote transthyretin degradation. The process produced no changes in pH, and the resulting temperature increases were < 1 °C. We conclude that the physical effects of PEFs, rather than thermal or chemical effects, facilitate aggregate degradation.

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