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Daubon T, Guyon J, Raymond AA, et al. The invasive proteome of glioblastoma revealed by laser-capture microdissection. Neurooncol Adv. 2019;1(1):vdz029doi: 10.1093/noajnl/vdz029.
Daubon, T., Guyon, J., Raymond, A. A., Dartigues, B., Rudewicz, J., Ezzoukhry, Z., Dupuy, J. W., Herbert, J. M. J., Saltel, F., Bjerkvig, R., Nikolski, M., & Bikfalvi, A. (2019). The invasive proteome of glioblastoma revealed by laser-capture microdissection. Neuro-oncology advances, 1(1), vdz029. https://doi.org/10.1093/noajnl/vdz029
Daubon, Thomas, et al. "The invasive proteome of glioblastoma revealed by laser-capture microdissection." Neuro-oncology advances vol. 1,1 (2019): vdz029. doi: https://doi.org/10.1093/noajnl/vdz029
Daubon T, Guyon J, Raymond AA, Dartigues B, Rudewicz J, Ezzoukhry Z, Dupuy JW, Herbert JMJ, Saltel F, Bjerkvig R, Nikolski M, Bikfalvi A. The invasive proteome of glioblastoma revealed by laser-capture microdissection. Neurooncol Adv. 2019 Sep 28;1(1):vdz029. doi: 10.1093/noajnl/vdz029. eCollection 2019. PMID: 32642662; PMCID: PMC7212852.
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Neurooncol Adv. 2019 Sep 28;1(1):vdz029. doi: 10.1093/noajnl/vdz029. eCollection 2019.

The invasive proteome of glioblastoma revealed by laser-capture microdissection.

Neuro-oncology advances

Thomas Daubon, Joris Guyon, Anne-Aurélie Raymond, Benjamin Dartigues, Justine Rudewicz, Zakaria Ezzoukhry, Jean-William Dupuy, John M J Herbert, Frédéric Saltel, Rolf Bjerkvig, Macha Nikolski, Andreas Bikfalvi

Affiliations

  1. INSERM U1029, Pessac, France.
  2. LAMC, University of Bordeaux, Bordeaux, France.
  3. KG Jebsen Brain Tumour Research Center, University of Bergen, Bergen, Norway.
  4. Oncoprot, TBM Core US005 University of Bordeaux, France.
  5. Bordeaux Bioinformatics Center, CBiB University of Bordeaux, France.
  6. Plateforme Protéome, University of Bordeaux, Bordeaux, France.
  7. Disease Gene Discovery Limited, London, UK.
  8. University Bordeaux, INSERM UMR1053, BaRITOn Bordeaux Research in Translational Oncology, Bordeaux, France.
  9. NorLux Neuro-Oncology, Department of Biomedicine University of Bergen, Norway.
  10. Oncology Department, Luxembourg Institute of Health 84, Val Fleuri, Luxembourg.
  11. LaBRI, UMR5800 University of Bordeaux, Talence, France.

PMID: 32642662 PMCID: PMC7212852 DOI: 10.1093/noajnl/vdz029

Abstract

BACKGROUND: Glioblastomas are heterogeneous tumors composed of a necrotic and tumor core and an invasive periphery.

METHODS: Here, we performed a proteomics analysis of laser-capture micro-dissected glioblastoma core and invasive areas of patient-derived xenografts.

RESULTS: Bioinformatics analysis identified enriched proteins in central and invasive tumor areas. Novel markers of invasion were identified, the genes proteolipid protein 1 (PLP1) and Dynamin-1 (DNM1), which were subsequently validated in tumors and by functional assays.

CONCLUSIONS: In summary, our results identify new networks and molecules that may play an important role in glioblastoma development and may constitute potential novel therapeutic targets.

© The Author(s) 2019. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology.

Keywords: glioblastoma; intratumor heterogeneity; invasion; patient-derived xenograft; proteomics analysis

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