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Morell M, Vogl AW, IJsseldijk LL, et al. Echolocating Whales and Bats Express the Motor Protein Prestin in the Inner Ear: A Potential Marker for Hearing Loss. Front Vet Sci. 2020;7:429doi: 10.3389/fvets.2020.00429.
Morell, M., Vogl, A. W., IJsseldijk, L. L., Piscitelli-Doshkov, M., Tong, L., Ostertag, S., Ferreira, M., Fraija-Fernandez, N., Colegrove, K. M., Puel, J. L., Raverty, S. A., & Shadwick, R. E. (2020). Echolocating Whales and Bats Express the Motor Protein Prestin in the Inner Ear: A Potential Marker for Hearing Loss. Frontiers in veterinary science, 7429. https://doi.org/10.3389/fvets.2020.00429
Morell, Maria, et al. "Echolocating Whales and Bats Express the Motor Protein Prestin in the Inner Ear: A Potential Marker for Hearing Loss." Frontiers in veterinary science vol. 7 (2020): 429. doi: https://doi.org/10.3389/fvets.2020.00429
Morell M, Vogl AW, IJsseldijk LL, Piscitelli-Doshkov M, Tong L, Ostertag S, Ferreira M, Fraija-Fernandez N, Colegrove KM, Puel JL, Raverty SA, Shadwick RE. Echolocating Whales and Bats Express the Motor Protein Prestin in the Inner Ear: A Potential Marker for Hearing Loss. Front Vet Sci. 2020 Jul 17;7:429. doi: 10.3389/fvets.2020.00429. eCollection 2020. PMID: 32851016; PMCID: PMC7396497.
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Front Vet Sci. 2020 Jul 17;7:429. doi: 10.3389/fvets.2020.00429. eCollection 2020.

Echolocating Whales and Bats Express the Motor Protein Prestin in the Inner Ear: A Potential Marker for Hearing Loss.

Frontiers in veterinary science

Maria Morell, A Wayne Vogl, Lonneke L IJsseldijk, Marina Piscitelli-Doshkov, Ling Tong, Sonja Ostertag, Marisa Ferreira, Natalia Fraija-Fernandez, Kathleen M Colegrove, Jean-Luc Puel, Stephen A Raverty, Robert E Shadwick

Affiliations

  1. Zoology Department, The University of British Columbia, Vancouver, BC, Canada.
  2. Inserm Unit 1051, Institute for Neurosciences of Montpellier, Montpellier, France.
  3. Institute for Terrestrial and Aquatic Wildlife Research, University of Veterinary Medicine Hannover, Foundation, Büsum, Germany.
  4. Department of Cellular and Physiological Sciences, Life Sciences Institute, The University of British Columbia, Vancouver, BC, Canada.
  5. Division of Pathology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands.
  6. Virginia Merrill Bloedel Hearing Research Center, University of Washington, Seattle, WA, United States.
  7. Department of Fisheries and Oceans Canada, Winnipeg, MB, Canada.
  8. Marine Animal Tissue Bank, Portuguese Wildlife Society, Estação de Campo de Quiaios, Figueira da Foz, Portugal.
  9. Centro Reabilitação Animais Marinhos, CPRAM, Ecomare, Estrada Do Porto de Pesca Costeira, Gafanha da Nazaré, Portugal.
  10. Marine Zoology Unit, Cavanilles Institute of Biodiversity and Evolutionary Biology, Science Park, University of Valencia, Valencia, Spain.
  11. Zoological Pathology Program, University of Illinois at Urbana-Champaign, Brookfield, IL, United States.
  12. Animal Health Center, Ministry of Agriculture, Abbotsford, BC, Canada.

PMID: 32851016 PMCID: PMC7396497 DOI: 10.3389/fvets.2020.00429

Abstract

Prestin is an integral membrane motor protein located in outer hair cells of the mammalian cochlea. It is responsible for electromotility and required for cochlear amplification. Although prestin works in a cycle-by-cycle mode up to frequencies of at least 79 kHz, it is not known whether or not prestin is required for the extreme high frequencies used by echolocating species. Cetaceans are known to possess a prestin coding gene. However, the expression and distribution pattern of the protein in the cetacean cochlea has not been determined, and the contribution of prestin to echolocation has not yet been resolved. Here we report the expression of the protein prestin in five species of echolocating whales and two species of echolocating bats. Positive labeling in the basolateral membrane of outer hair cells, using three anti-prestin antibodies, was found all along the cochlear spiral in echolocating species. These findings provide morphological evidence that prestin can have a role in cochlear amplification in the basolateral membrane up to 120-180 kHz. In addition, labeling of the cochlea with a combination of anti-prestin, anti-neurofilament, anti-myosin VI and/or phalloidin and DAPI will be useful for detecting potential recent cases of noise-induced hearing loss in stranded cetaceans. This study improves our understanding of the mechanisms involved in sound transduction in echolocating mammals, as well as describing an optimized methodology for detecting cases of hearing loss in stranded marine mammals.

Copyright © 2020 Morell, Vogl, IJsseldijk, Piscitelli-Doshkov, Tong, Ostertag, Ferreira, Fraija-Fernandez, Colegrove, Puel, Raverty and Shadwick.

Keywords: bat; echolocation; hair cells; immunofluorescence; inner ear; noise-induced hearing loss; prestin; whale

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