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Zheng J, Ye C, Hu B, et al. Bile acid profiles in bile and feces of obese mice by a high-performance liquid chromatography-tandem mass spectrometry. Biotechnol Appl Biochem. 2020;doi: 10.1002/bab.2055.
Zheng, J., Ye, C., Hu, B., Yang, H., Yao, Q., Ma, J., Liu, Y., & Liu, H. (2020). Bile acid profiles in bile and feces of obese mice by a high-performance liquid chromatography-tandem mass spectrometry. Biotechnology and applied biochemistry, . https://doi.org/10.1002/bab.2055
Zheng, Junping, et al. "Bile acid profiles in bile and feces of obese mice by a high-performance liquid chromatography-tandem mass spectrometry." Biotechnology and applied biochemistry vol. (2020). doi: https://doi.org/10.1002/bab.2055
Zheng J, Ye C, Hu B, Yang H, Yao Q, Ma J, Liu Y, Liu H. Bile acid profiles in bile and feces of obese mice by a high-performance liquid chromatography-tandem mass spectrometry. Biotechnol Appl Biochem. 2020 Nov 02; doi: 10.1002/bab.2055. Epub 2020 Nov 02. PMID: 33140486.
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Biotechnol Appl Biochem. 2020 Nov 02; doi: 10.1002/bab.2055. Epub 2020 Nov 02.

Bile acid profiles in bile and feces of obese mice by a high-performance liquid chromatography-tandem mass spectrometry.

Biotechnology and applied biochemistry

Junping Zheng, Cheng Ye, Baifei Hu, Huabing Yang, Qunfeng Yao, Jun Ma, Yang Liu, Hongtao Liu

Affiliations

  1. College of Life Sciences, Wuchang University of Technology, Wuhan, People's Republic of China.
  2. College of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan, People's Republic of China.
  3. Wuhan Customs Technology Center, Wuhan, People's Republic of China.

PMID: 33140486 DOI: 10.1002/bab.2055

Abstract

Bile acids (BAs) play a pivotal role in manipulating the development of metabolic diseases. However, due to the compositional complexity and functional variation of BAs, it remains unclear about the changes in BA pool for individuals with obesity or metabolic syndrome. We established a high-performance liquid chromatography-mass spectrometer detection system for the simultaneous analysis of both unconjugated and conjugated BAs in the bile and feces of mice. Ten BAs were completely separated, identified, and quantified with low limit of detection (0.5 ng/mL) and inter/intraday precision (relative standard deviation < 12%). By using this method, these BAs in bile and feces of mice were quantified. The result showed that taurochenodeoxycholic acid, taurine-conjugated α-muricholic acids, and taurine-conjugated β-muricholic acids were the dominated BAs in bile, whereas deoxycholic acid and chenodeoxycholic acid predominated in feces. Further, most of the BA levels were significantly elevated in either bile or fecal samples of high-fat diet-fed mice as compared with those in normal chow diet-fed mice, indicating that excessive production of BAs was closely associated with the occurrence of lipid metabolism disorders. In summary, the present method is practicable for analysis of BAs in bile and fecal samples of patients with obesity.

© 2020 International Union of Biochemistry and Molecular Biology, Inc.

Keywords: HPLC-MS/MS; bile acids; feces; high-fat diet; obesity

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