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Lucere KM, O'Malley MMR, Diermeier SD. Functional Screening Techniques to Identify Long Non-Coding RNAs as Therapeutic Targets in Cancer. Cancers (Basel). 2020;12(12)doi: 10.3390/cancers12123695.
Lucere, K. M., O'Malley, M. M. R., & Diermeier, S. D. (2020). Functional Screening Techniques to Identify Long Non-Coding RNAs as Therapeutic Targets in Cancer. Cancers, 12(12), . https://doi.org/10.3390/cancers12123695
Lucere, Kathleen M, et al. "Functional Screening Techniques to Identify Long Non-Coding RNAs as Therapeutic Targets in Cancer." Cancers vol. 12,12 (2020). doi: https://doi.org/10.3390/cancers12123695
Lucere KM, O'Malley MMR, Diermeier SD. Functional Screening Techniques to Identify Long Non-Coding RNAs as Therapeutic Targets in Cancer. Cancers (Basel). 2020 Dec 09;12(12). doi: 10.3390/cancers12123695. PMID: 33317042; PMCID: PMC7763270.
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Cancers (Basel). 2020 Dec 09;12(12). doi: 10.3390/cancers12123695.

Functional Screening Techniques to Identify Long Non-Coding RNAs as Therapeutic Targets in Cancer.

Cancers

Kathleen M Lucere, Megan M R O'Malley, Sarah D Diermeier

Affiliations

  1. Department of Biochemistry, University of Otago, Dunedin 9016, New Zealand.

PMID: 33317042 PMCID: PMC7763270 DOI: 10.3390/cancers12123695

Abstract

Recent technological advancements such as CRISPR/Cas-based systems enable multiplexed, high-throughput screening for new therapeutic targets in cancer. While numerous functional screens have been performed on protein-coding genes to date, long non-coding RNAs (lncRNAs) represent an emerging class of potential oncogenes and tumor suppressors, with only a handful of large-scale screens performed thus far. Here, we review in detail currently available screening approaches to identify new lncRNA drivers of tumorigenesis and tumor progression. We discuss the various approaches of genomic and transcriptional targeting using CRISPR/Cas9, as well as methods to post-transcriptionally target lncRNAs via RNA interference (RNAi), antisense oligonucleotides (ASOs) and CRISPR/Cas13. We discuss potential advantages, caveats and future applications of each method to provide an overview and guide on investigating lncRNAs as new therapeutic targets in cancer.

Keywords: CRISPR; antisense oligonucleotide (ASO); cancer; functional screening; long non-coding RNA (lncRNA); oncology; shRNA; siRNA; target identification; therapeutic target

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