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Kacal M, Zhang B, Hao Y, et al. Quantitative proteomic analysis of temporal lysosomal proteome and the impact of the KFERQ-like motif and LAMP2A in lysosomal targeting. Autophagy. 2021;17(11):3865-3874doi: 10.1080/15548627.2021.1876343.
Kacal, M., Zhang, B., Hao, Y., Norberg, E., & Vakifahmetoglu-Norberg, H. (2021). Quantitative proteomic analysis of temporal lysosomal proteome and the impact of the KFERQ-like motif and LAMP2A in lysosomal targeting. Autophagy, 17(11), 3865-3874. https://doi.org/10.1080/15548627.2021.1876343
Kacal, Merve, et al. "Quantitative proteomic analysis of temporal lysosomal proteome and the impact of the KFERQ-like motif and LAMP2A in lysosomal targeting." Autophagy vol. 17,11 (2021): 3865-3874. doi: https://doi.org/10.1080/15548627.2021.1876343
Kacal M, Zhang B, Hao Y, Norberg E, Vakifahmetoglu-Norberg H. Quantitative proteomic analysis of temporal lysosomal proteome and the impact of the KFERQ-like motif and LAMP2A in lysosomal targeting. Autophagy. 2021 Nov;17(11):3865-3874. doi: 10.1080/15548627.2021.1876343. Epub 2021 Jan 26. PMID: 33446043; PMCID: PMC8632328.
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Autophagy. 2021 Nov;17(11):3865-3874. doi: 10.1080/15548627.2021.1876343. Epub 2021 Jan 26.

Quantitative proteomic analysis of temporal lysosomal proteome and the impact of the KFERQ-like motif and LAMP2A in lysosomal targeting.

Autophagy

Merve Kacal, Boxi Zhang, Yuqing Hao, Erik Norberg, Helin Vakifahmetoglu-Norberg

Affiliations

  1. Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.

PMID: 33446043 PMCID: PMC8632328 DOI: 10.1080/15548627.2021.1876343

Abstract

Autophagic pathways are regulated mechanisms that play important roles in lysosome-mediated cellular degradation. Yet, the contribution of different autophagic pathways in lysosomal targeting, and characterization of the extent and specificity in their degradome remains largely uncharacterized. By undertaking a multiplex quantitative mass spectrometry approach, we have previously analyzed the lysosomal proteome during chaperone-mediated autophagy (CMA)-stimulated conditions in cancer cells. Here, we have extended our multiplex quantitative mass spectrometry and bioinformatics analysis on the proteome from isolated lysosomes to gain a comprehensive view of the temporal enriched lysosomal content upon non-macroautophagy-activated conditions. In parallel, we describe the functional dependency of LAMP2A on, and to what degree the presence of KFERQ-like motifs in proteins influences, their lysosomal targeting. These findings establish a framework for a better understanding of the degradome mediated by autophagic pathways beyond macroautophagy, and present characterization of the impact of LAMP2A in lysosomal targeting in cancer cells.

Keywords: Autophagy; cancer; chaperone-mediated autophagy; lysosome; proteomics

References

  1. Autophagy. 2016 Nov;12(11):1984-1999 - PubMed
  2. Nat Commun. 2015 Apr 16;6:6823 - PubMed
  3. J Cell Biol. 2018 Oct 1;217(10):3640-3655 - PubMed
  4. Cell. 2017 Feb 9;168(4):657-669 - PubMed
  5. Autophagy. 2019 Sep;15(9):1558-1571 - PubMed
  6. J Clin Invest. 2015 Jan;125(1):5-13 - PubMed
  7. Semin Cell Dev Biol. 2010 Sep;21(7):719-26 - PubMed
  8. Curr Opin Cell Biol. 1992 Apr;4(2):267-73 - PubMed
  9. Mol Cell Proteomics. 2008 Dec;7(12):2419-28 - PubMed
  10. J Cell Biol. 2015 Aug 31;210(5):705-16 - PubMed
  11. J Biol Chem. 2018 Apr 13;293(15):5414-5424 - PubMed
  12. Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4201-6 - PubMed
  13. Annu Rev Genet. 2009;43:67-93 - PubMed
  14. Mol Cell. 2010 Oct 22;40(2):280-93 - PubMed
  15. Genes Dev. 2013 Aug 1;27(15):1718-30 - PubMed
  16. Trends Biochem Sci. 1990 Aug;15(8):305-9 - PubMed
  17. Blood. 2018 Apr 12;131(15):1639-1653 - PubMed
  18. Nucleic Acids Res. 2015 Apr 20;43(7):e47 - PubMed
  19. PLoS Biol. 2019 May 31;17(5):e3000301 - PubMed
  20. Cell. 2011 Nov 11;147(4):728-41 - PubMed
  21. Dev Cell. 2011 Jan 18;20(1):131-9 - PubMed
  22. PLoS One. 2017 May 1;12(5):e0176234 - PubMed
  23. Mol Cell Biol. 2002 Aug;22(15):5575-84 - PubMed
  24. Nat Rev Mol Cell Biol. 2018 Jun;19(6):365-381 - PubMed
  25. Cell. 2011 Sep 30;147(1):223-34 - PubMed
  26. Autophagy. 2017 Jun 3;13(6):1064-1075 - PubMed
  27. Cell Metab. 2014 Sep 2;20(3):417-32 - PubMed
  28. Mol Biosyst. 2016 Feb;12(2):477-9 - PubMed

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