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Ma Q, Srivastav SP, Gamez S, et al. A mosquito small RNA genomics resource reveals dynamic evolution and host responses to viruses and transposons. Genome Res. 2021;31(3):512-528doi: 10.1101/gr.265157.120.
Ma, Q., Srivastav, S. P., Gamez, S., Dayama, G., Feitosa-Suntheimer, F., Patterson, E. I., Johnson, R. M., Matson, E. M., Gold, A. S., Brackney, D. E., Connor, J. H., Colpitts, T. M., Hughes, G. L., Rasgon, J. L., Nolan, T., Akbari, O. S., & Lau, N. C. (2021). A mosquito small RNA genomics resource reveals dynamic evolution and host responses to viruses and transposons. Genome research, 31(3), 512-528. https://doi.org/10.1101/gr.265157.120
Ma, Qicheng, et al. "A mosquito small RNA genomics resource reveals dynamic evolution and host responses to viruses and transposons." Genome research vol. 31,3 (2021): 512-528. doi: https://doi.org/10.1101/gr.265157.120
Ma Q, Srivastav SP, Gamez S, Dayama G, Feitosa-Suntheimer F, Patterson EI, Johnson RM, Matson EM, Gold AS, Brackney DE, Connor JH, Colpitts TM, Hughes GL, Rasgon JL, Nolan T, Akbari OS, Lau NC. A mosquito small RNA genomics resource reveals dynamic evolution and host responses to viruses and transposons. Genome Res. 2021 Mar;31(3):512-528. doi: 10.1101/gr.265157.120. Epub 2021 Jan 08. PMID: 33419731; PMCID: PMC7919454.
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Genome Res. 2021 Mar;31(3):512-528. doi: 10.1101/gr.265157.120. Epub 2021 Jan 08.

A mosquito small RNA genomics resource reveals dynamic evolution and host responses to viruses and transposons.

Genome research

Qicheng Ma, Satyam P Srivastav, Stephanie Gamez, Gargi Dayama, Fabiana Feitosa-Suntheimer, Edward I Patterson, Rebecca M Johnson, Erik M Matson, Alexander S Gold, Douglas E Brackney, John H Connor, Tonya M Colpitts, Grant L Hughes, Jason L Rasgon, Tony Nolan, Omar S Akbari, Nelson C Lau

Affiliations

  1. Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA.
  2. Division of Biological Sciences, Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093, USA.
  3. Department of Microbiology and the National Emerging Infectious Disease Laboratory, Boston University School of Medicine, Boston, Massachusetts 02118, USA.
  4. Departments of Vector Biology and Tropical Disease Biology, Centre for Neglected Tropical Diseases, Liverpool School of Tropical Medicine, Liverpool L3 5QA, United Kingdom.
  5. Department of Entomology, Center for Infectious Disease Dynamics, and the Huck Institutes for the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802, USA.
  6. Department of Environmental Sciences, The Connecticut Agricultural Experiment Station, New Haven, Connecticut 06511, USA.
  7. Boston University Genome Science Institute and the National Emerging Infectious Disease Laboratory, Boston, Massachusetts 02118, USA.

PMID: 33419731 PMCID: PMC7919454 DOI: 10.1101/gr.265157.120

Abstract

Although mosquitoes are major transmission vectors for pathogenic arboviruses, viral infection has little impact on mosquito health. This immunity is caused in part by mosquito RNA interference (RNAi) pathways that generate antiviral small interfering RNAs (siRNAs) and Piwi-interacting RNAs (piRNAs). RNAi also maintains genome integrity by potently repressing mosquito transposon activity in the germline and soma. However, viral and transposon small RNA regulatory pathways have not been systematically examined together in mosquitoes. Therefore, we developed an integrated mosquito small RNA genomics (MSRG) resource that analyzes the transposon and virus small RNA profiles in mosquito cell cultures and somatic and gonadal tissues across four medically important mosquito species. Our resource captures both somatic and gonadal small RNA expression profiles within mosquito cell cultures, and we report the evolutionary dynamics of a novel Mosquito-Conserved piRNA Cluster Locus (MCpiRCL) made up of satellite DNA repeats. In the larger culicine mosquito genomes we detected highly regular periodicity in piRNA biogenesis patterns coinciding with the expansion of Piwi pathway genes. Finally, our resource enables detection of cross talk between piRNA and siRNA populations in mosquito cells during a response to virus infection. The MSRG resource will aid efforts to dissect and combat the capacity of mosquitoes to tolerate and spread arboviruses.

© 2021 Ma et al.; Published by Cold Spring Harbor Laboratory Press.

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