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Yan H, Xiang P, Zhang J, et al. Dynamic changes of serum protein in rats with acute intoxication of Chinese cobra snake venom by proteomic analysis. Forensic Sci Res. 2017;5(4):309-321doi: 10.1080/20961790.2017.1405565.
Yan, H., Xiang, P., Zhang, J., Xie, L., & Shen, M. (2017). Dynamic changes of serum protein in rats with acute intoxication of Chinese cobra snake venom by proteomic analysis. Forensic sciences research, 5(4), 309-321. https://doi.org/10.1080/20961790.2017.1405565
Yan, Hui, et al. "Dynamic changes of serum protein in rats with acute intoxication of Chinese cobra snake venom by proteomic analysis." Forensic sciences research vol. 5,4 (2017): 309-321. doi: https://doi.org/10.1080/20961790.2017.1405565
Yan H, Xiang P, Zhang J, Xie L, Shen M. Dynamic changes of serum protein in rats with acute intoxication of Chinese cobra snake venom by proteomic analysis. Forensic Sci Res. 2017 Dec 21;5(4):309-321. doi: 10.1080/20961790.2017.1405565. PMID: 33457049; PMCID: PMC7782176.
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Forensic Sci Res. 2017 Dec 21;5(4):309-321. doi: 10.1080/20961790.2017.1405565.

Dynamic changes of serum protein in rats with acute intoxication of Chinese cobra snake venom by proteomic analysis.

Forensic sciences research

Hui Yan, Ping Xiang, Jingshuo Zhang, Liqi Xie, Min Shen

Affiliations

  1. Shanghai Key Laboratory of Forensic Science, Shanghai Forensic Platform, Department of Forensic Toxicology, Academy of Forensic Science, Shanghai, China.
  2. College of Pharmaceutical Sciences, Soochow Universtity, Suzhou, Jiangsu, China.
  3. Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

PMID: 33457049 PMCID: PMC7782176 DOI: 10.1080/20961790.2017.1405565

Abstract

To elucidate the toxic mechanism of snake venom at the protein level, proteomics technology was applied to investigate the effect of venom on circulation in the mammalian body. Temporal proteomic analysis was performed to profile the dynamic changes in the sera of Sprague-Dawley rats administered with Chinese cobra venom or saline. Using 8-plex iTRAQ analysis, 392 and 636 serum proteins were identified to be linearly upregulated or downregulated over time in the low-dose group and high-dose group, respectively. These proteins were mainly associated with the acute phase response pathway, complement system, and liver X receptor (LXR)/retinoid X receptor (RXR) and farnesoid X receptor (FXR)/RXR activation pathways. Compared with the low-dose group, the immune response and integrin pathways were inhibited in the high-dose group, although no obvious effect was observed. With consistently higher or lower expression in the high-dose group compared to the low-dose group throughout the whole process of venom poisoning, two proteins, Kininogen-1 (KNG1) and orosomucoid 1 (ORM1), which are involved in metabolism and immune response, occupied a core position in the pathway network and are considered venom dose-dependent biomarker candidates.

© 2017 The Author(s). Published by Taylor & Francis Group on behalf of the Academy of Forensic Science.

Keywords: Forensic sciences; cobra; forensic toxicology; proteomics; rats; serum; snake bite

Conflict of interest statement

No potential conflict of interest was reported by the authors.

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