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Qiao L, Amhare AF, Deng H, et al. Protective effect of chondroitin sulfate nano-selenium on chondrocyte of patients with Kashin-Beck disease. J Biomater Appl. 2021;35(10):1347-1354doi: 10.1177/0885328220988427.
Qiao, L., Amhare, A. F., Deng, H., Lv, Y., Zhao, Y., Liu, J., Lei, J., Wang, L., Chilufya, M. M., & Han, J. (2021). Protective effect of chondroitin sulfate nano-selenium on chondrocyte of patients with Kashin-Beck disease. Journal of biomaterials applications, 35(10), 1347-1354. https://doi.org/10.1177/0885328220988427
Qiao, Lichun, et al. "Protective effect of chondroitin sulfate nano-selenium on chondrocyte of patients with Kashin-Beck disease." Journal of biomaterials applications vol. 35,10 (2021): 1347-1354. doi: https://doi.org/10.1177/0885328220988427
Qiao L, Amhare AF, Deng H, Lv Y, Zhao Y, Liu J, Lei J, Wang L, Chilufya MM, Han J. Protective effect of chondroitin sulfate nano-selenium on chondrocyte of patients with Kashin-Beck disease. J Biomater Appl. 2021 May;35(10):1347-1354. doi: 10.1177/0885328220988427. Epub 2021 Jan 24. PMID: 33487067.
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J Biomater Appl. 2021 May;35(10):1347-1354. doi: 10.1177/0885328220988427. Epub 2021 Jan 24.

Protective effect of chondroitin sulfate nano-selenium on chondrocyte of patients with Kashin-Beck disease.

Journal of biomaterials applications

Lichun Qiao, Abebe F Amhare, Huan Deng, Yizhen Lv, Yan Zhao, Jiaxin Liu, Jian Lei, Liyun Wang, Mumba M Chilufya, Jing Han

Affiliations

  1. School of Public Health, Health Science Center, 12480Xi'an Jiaotong University, Xi'an, China.

PMID: 33487067 DOI: 10.1177/0885328220988427

Abstract

OBJECTIVE: To investigate the protective effect of chondroitin sulfate nano-selenium (SeCS) on chondrocyte of Kashin-Beck disease (KBD).

METHODS: Chondrocyte samples were isolated from the cartilage of three male KBD patients (54-57 years old). The chondrocytes were respectively divided into four groups: (a) control group, (b) SeCS supplement group (100 ng/mL SeCS), (c) T-2 + SeCS supplement group (20 ng/mL T-2 + 100 ng/mL SeCS), and (d) T-2 group (20 ng/mL T-2). Live/dead staining and transmission electron microscopy (TEM) were used to observe cell viability and ultrastructural changes in chondrocytes respectively. Expressions of Caspase-9, cytochrome C (Cyt-C), and chondroitin sulfate (CS) structure-modifying sulfotransferases including carbohydrate sulfotransferase 3, 15 (CHST-3, CHST-15), and uronyl 2-O-sulfotransferase (UST) were examined by quantitative real-time polymerase chain reaction.

RESULTS: After one- or three-days intervention, the number of living chondrocytes in the SeCS supplement group was higher than that in the control group, while it is opposite in the T-2 + SeCS supplement group and T-2 group. The cellular villi number in the surface increased in the SeCS supplement group compared with the control group. Mitochondrial morphology density was improved in the T-2 + SeCS supplement group compared with the T-2 group. Expressions of CHST-3, CHST-15, UST, Caspase-9, and Cyt-C on the mRNA level significantly increased in the T-2 + SeCS supplement group and T-2 group compared with the control group.

CONCLUSIONS: SeCS supplement increased the number of living chondrocytes, improved the ultrastructure, and altered the expressions of CS structure-modifying sulfotransferases, Caspase-9, and Cyt-C.

Keywords: Chondroitin sulfate nano-selenium; Kashin-Beck disease; chondrocyte; sulfotransferases

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