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Suarez-Fernandez M, Sambles C, Lopez-Moya F, et al. Chitosan modulates Pochonia chlamydosporia gene expression during nematode egg parasitism. Environ Microbiol. 2021;23(9):4980-4997doi: 10.1111/1462-2920.15408.
Suarez-Fernandez, M., Sambles, C., Lopez-Moya, F., Nueda, M. J., Studholme, D. J., & Lopez-Llorca, L. V. (2021). Chitosan modulates Pochonia chlamydosporia gene expression during nematode egg parasitism. Environmental microbiology, 23(9), 4980-4997. https://doi.org/10.1111/1462-2920.15408
Suarez-Fernandez, Marta, et al. "Chitosan modulates Pochonia chlamydosporia gene expression during nematode egg parasitism." Environmental microbiology vol. 23,9 (2021): 4980-4997. doi: https://doi.org/10.1111/1462-2920.15408
Suarez-Fernandez M, Sambles C, Lopez-Moya F, Nueda MJ, Studholme DJ, Lopez-Llorca LV. Chitosan modulates Pochonia chlamydosporia gene expression during nematode egg parasitism. Environ Microbiol. 2021 Sep;23(9):4980-4997. doi: 10.1111/1462-2920.15408. Epub 2021 Feb 05. PMID: 33496078; PMCID: PMC8518118.
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Environ Microbiol. 2021 Sep;23(9):4980-4997. doi: 10.1111/1462-2920.15408. Epub 2021 Feb 05.

Chitosan modulates Pochonia chlamydosporia gene expression during nematode egg parasitism.

Environmental microbiology

Marta Suarez-Fernandez, Christine Sambles, Federico Lopez-Moya, María J Nueda, David J Studholme, Luis Vicente Lopez-Llorca

Affiliations

  1. Laboratory of Plant Pathology, Department of Marine Sciences and Applied Biology, University of Alicante, Alicante, 03080, Spain.
  2. Multidisciplinary Institute for Environmental Studies (MIES) Ramon Margalef, University of Alicante, Alicante, 03080, Spain.
  3. School of Biosciences, University of Exeter, Exeter, UK.
  4. Mathematics Department, University of Alicante, Alicante, 03080, Spain.

PMID: 33496078 PMCID: PMC8518118 DOI: 10.1111/1462-2920.15408

Abstract

Climate change makes plant-parasitic nematodes (PPN) an increasing threat to commercial crops. PPN can be managed sustainably by the biocontrol fungus Pochonia chlamydosporia (Pc). Chitosan generated from chitin deacetylation enhances PPN parasitism by Pc. In this work, we investigate the molecular mechanisms of Pc for chitosan resistance and root-knot nematode (RKN) parasitism, using transcriptomics. Chitosan and RKN modify the expression of Pc genes, mainly those involved in oxidation-reduction processes. Both agents significantly modify the expression of genes associated to 113 GO terms and 180 Pc genes. Genes encoding putative glycoproteins (Pc adhesives) to nematode eggshell, as well as genes involved in redox, carbohydrate and lipid metabolism trigger the response to chitosan. We identify genes expressed in both the parasitic and endophytic phases of the Pc lifecycle; these include proteases, chitosanases and transcription factors. Using the Pathogen-Host Interaction database (PHI-base), our previous RNA-seq data and RT-PCR of Pc colonizing banana we have investigated genes expressed both in the parasitic and endophytic phases of Pc lifecycle.

© 2021 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

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