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Boateng BO, Elcoroaristizabal S, Ryder AG. Development of a rapid polarized total synchronous fluorescence spectroscopy (pTSFS) method for protein quantification in a model bioreactor broth. Biotechnol Bioeng. 2021;118(5):1805-1817doi: 10.1002/bit.27694.
Boateng, B. O., Elcoroaristizabal, S., & Ryder, A. G. (2021). Development of a rapid polarized total synchronous fluorescence spectroscopy (pTSFS) method for protein quantification in a model bioreactor broth. Biotechnology and bioengineering, 118(5), 1805-1817. https://doi.org/10.1002/bit.27694
Boateng, Bernard O, et al. "Development of a rapid polarized total synchronous fluorescence spectroscopy (pTSFS) method for protein quantification in a model bioreactor broth." Biotechnology and bioengineering vol. 118,5 (2021): 1805-1817. doi: https://doi.org/10.1002/bit.27694
Boateng BO, Elcoroaristizabal S, Ryder AG. Development of a rapid polarized total synchronous fluorescence spectroscopy (pTSFS) method for protein quantification in a model bioreactor broth. Biotechnol Bioeng. 2021 May;118(5):1805-1817. doi: 10.1002/bit.27694. Epub 2021 Feb 04. PMID: 33501639.
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Biotechnol Bioeng. 2021 May;118(5):1805-1817. doi: 10.1002/bit.27694. Epub 2021 Feb 04.

Development of a rapid polarized total synchronous fluorescence spectroscopy (pTSFS) method for protein quantification in a model bioreactor broth.

Biotechnology and bioengineering

Bernard O Boateng, Saioa Elcoroaristizabal, Alan G Ryder

Affiliations

  1. Nanoscale BioPhotonics Laboratory, School of Chemistry, National University of Ireland, Galway, Ireland.

PMID: 33501639 DOI: 10.1002/bit.27694

Abstract

Protein quantification during bioprocess monitoring is essential for biopharmaceutical manufacturing and is complicated by the complex chemical composition of the bioreactor broth. Here we present the early-stage development and optimization of a polarized total synchronous fluorescence spectroscopy (pTSFS) method for protein quantification in a hydrolysate-protein model (mimics clarified bioreactor broth samples) using a standard benchtop laboratory fluorometer. We used UV transmitting polarizers to provide wider range pTSFS spectra for screening of the four different TSFS spectra generated by the measurement: parallel (||), perpendicular (⊥), unpolarized (T) intensity spectra and anisotropy maps. TSFS

© 2021 Wiley Periodicals LLC.

Keywords: bioprocess monitoring; chemometrics; multidimensional fluorescence; polarization; process analytical technology; protein

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