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An excitation emission fluorescence lifetime spectrometer using a frequency doubled supercontinuum laser source.

Methods and applications in fluorescence

Melnikau D, Elcoroaristizabal S, Ryder AG.
PMID: 30101757
Methods Appl Fluoresc. 2018 Sep 03;6(4):045007. doi: 10.1088/2050-6120/aad9ae.

The accurate fluorescence analysis of complex, multi-fluorophore containing proteins requires the use of multi-dimensional measurement techniques. For the measurement of intrinsic fluorescence from tyrosine (Tyr) and tryptophan (Trp) one needs tuneable UV excitation and for steady-state measurements like Excitation...

Development of a rapid polarized total synchronous fluorescence spectroscopy (pTSFS) method for protein quantification in a model bioreactor broth.

Biotechnology and bioengineering

Boateng BO, Elcoroaristizabal S, Ryder AG.
PMID: 33501639
Biotechnol Bioeng. 2021 May;118(5):1805-1817. doi: 10.1002/bit.27694. Epub 2021 Feb 04.

Protein quantification during bioprocess monitoring is essential for biopharmaceutical manufacturing and is complicated by the complex chemical composition of the bioreactor broth. Here we present the early-stage development and optimization of a polarized total synchronous fluorescence spectroscopy (pTSFS) method...

Fatty acid synthase methylation levels in adipose tissue: effects of an obesogenic diet and phenol compounds.

Genes & nutrition

Gracia A, Elcoroaristizabal X, Fernández-Quintela A, Miranda J, Bediaga NG, M de Pancorbo M, Rimando AM, Portillo MP.
PMID: 24903834
Genes Nutr. 2014 Jul;9(4):411. doi: 10.1007/s12263-014-0411-9. Epub 2014 Jun 06.

DNA methylation is an epigenetic mechanism that can inhibit gene transcription. The aim of this study was to assess changes induced by an obesogenic diet in the methylation profile of genes involved in adipose tissue triacylglycerol metabolism, and to...

Inflammatory gene expression profiling in peripheral blood from patients with Alzheimer's disease reveals key pathways and hub genes with potential diagnostic utility: a preliminary study.

PeerJ

Cardona K, Medina J, Orrego-Cardozo M, Restrepo de Mejía F, Elcoroaristizabal X, Naranjo Galvis CA.
PMID: 34484988
PeerJ. 2021 Aug 20;9:e12016. doi: 10.7717/peerj.12016. eCollection 2021.

BACKGROUND: Alzheimer's disease (AD) is an age-related neurodegenerative disease caused by central nervous system disorders. Late-onset Alzheimer disease (LOAD) is the most common neurodegenerative disorder worldwide. Differences at the expression level of certain genes, resulting from either genetic variations...

Hyperspectral image analysis. A tutorial.

Analytica chimica acta

Amigo JM, Babamoradi H, Elcoroaristizabal S.
PMID: 26481986
Anal Chim Acta. 2015 Oct 08;896:34-51. doi: 10.1016/j.aca.2015.09.030. Epub 2015 Sep 25.

This tutorial aims at providing guidelines and practical tools to assist with the analysis of hyperspectral images. Topics like hyperspectral image acquisition, image pre-processing, multivariate exploratory analysis, hyperspectral image resolution, classification and final digital image processing will be exposed,...

Showing 1 to 5 of 5 entries