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Li W, Fan Z, Lin Y, et al. Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells. Front Bioeng Biotechnol. 2021;9:646363doi: 10.3389/fbioe.2021.646363.
Li, W., Fan, Z., Lin, Y., & Wang, T. Y. (2021). Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells. Frontiers in bioengineering and biotechnology, 9646363. https://doi.org/10.3389/fbioe.2021.646363
Li, Weifeng, et al. "Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells." Frontiers in bioengineering and biotechnology vol. 9 (2021): 646363. doi: https://doi.org/10.3389/fbioe.2021.646363
Li W, Fan Z, Lin Y, Wang TY. Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells. Front Bioeng Biotechnol. 2021 Mar 15;9:646363. doi: 10.3389/fbioe.2021.646363. eCollection 2021. PMID: 33791287; PMCID: PMC8006267.
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Front Bioeng Biotechnol. 2021 Mar 15;9:646363. doi: 10.3389/fbioe.2021.646363. eCollection 2021.

Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells.

Frontiers in bioengineering and biotechnology

Weifeng Li, Zhenlin Fan, Yan Lin, Tian-Yun Wang

Affiliations

  1. Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, China.
  2. International Joint Research Laboratory for Recombinant Pharmaceutical Protein Expression System of Henan, Xinxiang Medical University, Xinxiang, China.

PMID: 33791287 PMCID: PMC8006267 DOI: 10.3389/fbioe.2021.646363

Abstract

At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs. In this review, the history and key components of SFM are first summarized, and its preparation and experimental design are described. Some small molecule compound additives can improve the yield and quality of RTP. The function and possible mechanisms of these additives are also reviewed here. Finally, the future perspectives of SFM use with CHO cells for RTP production are discussed.

Copyright © 2021 Li, Fan, Lin and Wang.

Keywords: Chinese hamster ovary cells; cell culture; glycosylation; recombinant therapeutic protein; serum free medium

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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