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STAR Protoc. 2021 Mar 23;2(2):100405. doi: 10.1016/j.xpro.2021.100405. eCollection 2021 Jun 18.

Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons.

STAR protocols

Xiling Yin, Jungwoo Wren Kim, Shiyu Liu, Ted M Dawson, Valina L Dawson

Affiliations

  1. Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  2. Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  3. Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  4. Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  5. Soloman H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  6. Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  7. Adrienne Helis Malvin Medical Research Foundation, New Orleans, LA 70130, USA.
  8. Diana Helis Henry Medical Research Foundation, New Orleans, LA 70130, USA.

PMID: 33855307 PMCID: PMC8024766 DOI: 10.1016/j.xpro.2021.100405

Abstract

Calcium regulation is a critical process in neurons, and Ca

© 2021 The Author(s).

Keywords: Cell Differentiation; Microscopy; Neuroscience; Stem Cells

Conflict of interest statement

The authors declare no competing interests.

References

  1. Yakubutsu Seishin Kodo. 1992 Apr;12(2):67-73 - PubMed
  2. Cell Stem Cell. 2013 Mar 7;12(3):354-67 - PubMed
  3. Methods Mol Biol. 2013;998:149-57 - PubMed
  4. Cell Stem Cell. 2020 Oct 1;27(4):633-645.e7 - PubMed

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