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Han L, Park D, Reddy A, et al. Comparing endocervical mucus proteome of humans and rhesus macaques. Proteomics Clin Appl. 2021;15(4):e2100023doi: 10.1002/prca.202100023.
Han, L., Park, D., Reddy, A., Wilmarth, P. A., & Jensen, J. T. (2021). Comparing endocervical mucus proteome of humans and rhesus macaques. Proteomics. Clinical applications, 15(4), e2100023. https://doi.org/10.1002/prca.202100023
Han, Leo, et al. "Comparing endocervical mucus proteome of humans and rhesus macaques." Proteomics. Clinical applications vol. 15,4 (2021): e2100023. doi: https://doi.org/10.1002/prca.202100023
Han L, Park D, Reddy A, Wilmarth PA, Jensen JT. Comparing endocervical mucus proteome of humans and rhesus macaques. Proteomics Clin Appl. 2021 Jul;15(4):e2100023. doi: 10.1002/prca.202100023. Epub 2021 May 26. PMID: 33991406; PMCID: PMC8653767.
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Proteomics Clin Appl. 2021 Jul;15(4):e2100023. doi: 10.1002/prca.202100023. Epub 2021 May 26.

Comparing endocervical mucus proteome of humans and rhesus macaques.

Proteomics. Clinical applications

Leo Han, Daye Park, Ashok Reddy, Phillip A Wilmarth, Jeffrey T Jensen

Affiliations

  1. Oregon Health & Science University, Department of Obstetrics and Gynecology, Portland, Oregon, USA.
  2. Oregon National Primate Research Center, Division of Reproductive and Developmental Sciences, Portland, Oregon, USA.
  3. Augusta University/University of Georgia Medical Partnership, Athens, Georgia, USA.
  4. Proteomic Shared Resource, Oregon Health & Science University, Portland, Oregon, USA.

PMID: 33991406 PMCID: PMC8653767 DOI: 10.1002/prca.202100023

Abstract

PURPOSE: Endocervical mucus changes play a key role in regulating fertility throughout the menstrual cycle and in response to hormonal contraceptives. Non-human primates (NHP) provide the most translational animal model for reproductive tract studies, as they have hormonally-regulated menstrual cycles and mucus changes, similar to women.

EXPERIMENTAL DESIGN: We used TMT labelling and LC-LC/MS to compare the proteins found in the mucus of the rhesus macaque to the mucus of the human endocervix. Data are available via ProteomeXchange with identifier PXD021710.

RESULTS: We found 3048 total proteins present in both rhesus mucus and human mucus, and of these, 57% showed a similar expression pattern. An even higher similarity occurred in the top 500 most prevalent proteins, with overlap in 341 (68%) proteins. Mucin MUC5B was the most highly expressed mucin protein (top 10 expressed proteins in both) but other key proteins related to mucus structure were present in both samples.

CONCLUSIONS AND CLINICAL RELEVANCE: We find that the mucus proteome of the endocervical mucus is highly conserved in NHP and women. This supports use of the NHP model system for studies of the endocervix and trials of novel fertility treatments targeting the cervix.

© 2021 Wiley-VCH GmbH.

Keywords: endocervix; mucus; primate; proteome; rhesus macaque

References

  1. Eur J Contracept Reprod Health Care. 2019 Jun;24(3):209-215 - PubMed
  2. Hum Reprod Update. 2017 May 1;23(3):289-299 - PubMed
  3. J Clin Endocrinol Metab. 2001 Feb;86(2):594-600 - PubMed
  4. Anal Chem. 2014 Jul 15;86(14):7150-8 - PubMed
  5. J Ocul Biol Dis Infor. 2009 Dec 12;2(4):223-234 - PubMed
  6. Bioinformatics. 2010 Jan 1;26(1):139-40 - PubMed
  7. Mol Biosyst. 2015 Jun;11(6):1717-25 - PubMed
  8. Eur J Obstet Gynecol Reprod Biol. 2011 Apr;155(2):157-60 - PubMed
  9. J Proteome Res. 2014 Apr 4;13(4):1885-95 - PubMed
  10. Mol Cell Proteomics. 2007 Apr;6(4):708-16 - PubMed
  11. Hum Reprod. 1996 Oct;11 Suppl 2:150-64 - PubMed
  12. Front Biosci. 2001 Oct 01;6:D1245-55 - PubMed
  13. Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450 - PubMed
  14. Contraception. 2017 Nov;96(5):310-321 - PubMed
  15. Proteomics. 2013 Jan;13(1):22-4 - PubMed

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