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Foods. 2021 May 15;10(5). doi: 10.3390/foods10051097.

Impact of Extraction Method on the Detection of Quality Biomarkers in Normal vs. DFD Meat.

Foods (Basel, Switzerland)

Laura González-Blanco, Yolanda Diñeiro, Andrea Díaz-Luis, Ana Coto-Montes, Mamen Oliván, Verónica Sierra

Affiliations

  1. Área de Sistemas de Producción Animal, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Ctra. AS-267, PK 19, 33300 Villaviciosa, Asturias, Spain.
  2. Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Av. del Hospital Universitario, s/n, 33011 Oviedo, Asturias, Spain.
  3. Department of Molecular Biology, Faculty of Medicine, University of Cantabria, Av. Herrera Oria, s/n, 39011 Santander, Cantabria, Spain.
  4. Department of Morphology and Cell Biology, Faculty of Medicine, University of Oviedo, Av. Julián Clavería, 6, 33006 Oviedo, Asturias, Spain.

PMID: 34063396 PMCID: PMC8156798 DOI: 10.3390/foods10051097

Abstract

The objective of this work was to demonstrate how the extraction method affects the reliability of biomarker detection and how this detection depends on the biomarker location within the cell compartment. Different extraction methods were used to study the sarcoplasmic and myofibrillar fractions of the

Keywords: DFD meat; cellular compartments; meat quality biomarkers; myofibrillar proteins; oxidative stress; protein extractability; proteomics; sarcoplasmic proteins

References

  1. Electrophoresis. 1998 May;19(5):837-44 - PubMed
  2. Muscle Nerve. 2000 Nov;23(11):1647-66 - PubMed
  3. Meat Sci. 2012 Feb;90(2):345-51 - PubMed
  4. Meat Sci. 2011 Jun;88(2):261-70 - PubMed
  5. Meat Sci. 2009 Nov;83(3):474-8 - PubMed
  6. Crit Rev Food Sci Nutr. 2017 Oct 13;57(15):3260-3280 - PubMed
  7. J Agric Food Chem. 2010 Jun 23;58(12):7408-14 - PubMed
  8. Proteome Sci. 2015 Feb 05;13:5 - PubMed
  9. J Anim Sci. 2009 Jul;87(7):2391-9 - PubMed
  10. Cell Mol Life Sci. 2004 Dec;61(24):3016-33 - PubMed
  11. Proteomics. 2002 Sep;2(9):1169-86 - PubMed
  12. Am J Physiol Cell Physiol. 2012 Sep 15;303(6):C654-65 - PubMed
  13. Meat Sci. 1981 May;5(3):223-32 - PubMed
  14. J Agric Food Chem. 2012 Mar 28;60(12):3196-203 - PubMed
  15. J Proteomics. 2020 Apr 30;218:103722 - PubMed
  16. Animal. 2017 Sep;11(9):1599-1607 - PubMed
  17. Free Radic Biol Med. 2017 Sep;110:31-41 - PubMed
  18. Meat Sci. 2018 Nov;145:209-219 - PubMed
  19. J Anim Sci. 2016 Jun;94(6):2583-91 - PubMed
  20. Genetics. 1979 Apr;91(4):723-42 - PubMed
  21. Food Chem. 2016 Apr 1;196:42-9 - PubMed
  22. Animal. 2015 Jul;9(7):1188-94 - PubMed
  23. Electrophoresis. 1998 May;19(5):758-60 - PubMed
  24. Meat Sci. 1998 Aug;49(4):447-57 - PubMed
  25. Poult Sci. 2014 Jul;93(7):1825-33 - PubMed
  26. Anal Biochem. 1976 May 7;72:248-54 - PubMed
  27. Compr Rev Food Sci Food Saf. 2017 May;16(3):400-430 - PubMed
  28. Meat Sci. 2004 Aug;67(4):697-703 - PubMed
  29. Food Res Int. 2020 Jan;127:108739 - PubMed
  30. Am J Physiol Regul Integr Comp Physiol. 2007 Aug;293(2):R844-53 - PubMed
  31. Proteomics. 2003 Aug;3(8):1408-17 - PubMed
  32. J Anim Sci. 2015 Jan;93(1):62-70 - PubMed

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