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You W, Li M, Qi Y, et al. CRISPR/Cas9-Mediated Specific Integration of . Genes (Basel). 2021;12(7)doi: 10.3390/genes12071027.
You, W., Li, M., Qi, Y., Wang, Y., Chen, Y., Liu, Y., Li, L., Ouyang, H., & Pang, D. (2021). CRISPR/Cas9-Mediated Specific Integration of . Genes, 12(7), . https://doi.org/10.3390/genes12071027
You, Wenni, et al. "CRISPR/Cas9-Mediated Specific Integration of ." Genes vol. 12,7 (2021). doi: https://doi.org/10.3390/genes12071027
You W, Li M, Qi Y, Wang Y, Chen Y, Liu Y, Li L, Ouyang H, Pang D. CRISPR/Cas9-Mediated Specific Integration of . Genes (Basel). 2021 Jul 01;12(7). doi: 10.3390/genes12071027. PMID: 34356043; PMCID: PMC8305104.
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Genes (Basel). 2021 Jul 01;12(7). doi: 10.3390/genes12071027.

CRISPR/Cas9-Mediated Specific Integration of .

Genes

Wenni You, Mengjing Li, Yilin Qi, Yanbing Wang, Yiwu Chen, Ying Liu, Li Li, Hongsheng Ouyang, Daxin Pang

Affiliations

  1. Key Lab for Zoonoses Research, Ministry of Education, Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China.
  2. Chongqing Research Institute, Jilin University, Chongqing 401123, China.

PMID: 34356043 PMCID: PMC8305104 DOI: 10.3390/genes12071027

Abstract

Many researchers have focused on knock-in pigs for site-specific integration, but little attention has been given to genetically modified pigs with the targeted integration of multiple recombinant genes. To establish a multigene targeted knock-in editing system, we used the internal ribosome entry site (IRES) and self-cleaving 2A peptide technology to construct a plasmid coexpressing the fatty acid desaturase (

Keywords: CRISPR/Cas9; Fat-1; IGF-1; knock-in; pig

References

  1. Nat Biotechnol. 2016 May 6;34(5):479-81 - PubMed
  2. FEBS J. 2018 Aug;285(15):2828-2839 - PubMed
  3. Science. 2002 Feb 8;295(5557):1089-92 - PubMed
  4. Nat Biomed Eng. 2021 Feb;5(2):134-143 - PubMed
  5. Nutrients. 2017 Feb 23;9(3): - PubMed
  6. Trends Immunol. 2005 Oct;26(10):535-42 - PubMed
  7. World J Surg. 2017 Jun;41(6):1584-1594 - PubMed
  8. Schizophr Res. 2017 Oct;188:110-117 - PubMed
  9. Cell Mol Life Sci. 2020 Feb;77(4):719-733 - PubMed
  10. Int J Cancer. 2016 May 1;138(9):2247-56 - PubMed
  11. G3 (Bethesda). 2018 May 4;8(5):1747-1754 - PubMed
  12. Am J Physiol. 1997 Dec;273(6):L1174-81 - PubMed
  13. Traffic. 2004 Aug;5(8):616-26 - PubMed
  14. Biogerontology. 2002;3(1-2):19-24 - PubMed
  15. Prostaglandins Other Lipid Mediat. 2017 Jan;128-129:34-42 - PubMed
  16. Zygote. 2016 Jun;24(3):442-56 - PubMed
  17. Nat Biotechnol. 2006 Apr;24(4):435-6 - PubMed
  18. Nature. 1985 Jun 20-26;315(6021):680-3 - PubMed
  19. Nat Cell Biol. 2001 Nov;3(11):1009-13 - PubMed
  20. Plant Biotechnol J. 2011 Aug;9(6):713-21 - PubMed
  21. Front Immunol. 2016 Sep 13;7:350 - PubMed
  22. Cell Reprogram. 2012 Jun;14(3):258-66 - PubMed
  23. Nat Genet. 2001 Feb;27(2):195-200 - PubMed
  24. Nat Cell Biol. 2001 Nov;3(11):1014-9 - PubMed
  25. Endocrinology. 1999 Nov;140(11):5178-84 - PubMed
  26. Expert Opin Biol Ther. 2005 May;5(5):627-38 - PubMed
  27. Theriogenology. 2009 Sep 1;72(4):535-41 - PubMed
  28. Sci Rep. 2017 Dec 7;7(1):17136 - PubMed
  29. Antiviral Res. 2020 Feb;174:104696 - PubMed
  30. PLoS One. 2014 Apr 09;9(4):e94356 - PubMed
  31. Cell Physiol Biochem. 2013;32(3):523-32 - PubMed
  32. PLoS Pathog. 2018 Dec 13;14(12):e1007193 - PubMed
  33. Heart Lung Circ. 2017 Aug;26(8):846-855 - PubMed
  34. Protein Cell. 2015 May;6(5):363-372 - PubMed
  35. Cell Death Dis. 2019 Oct 28;10(11):823 - PubMed
  36. Mol Cancer Ther. 2008 Oct;7(10):3203-11 - PubMed
  37. Annu Rev Physiol. 1985;47:443-67 - PubMed
  38. Genes (Basel). 2020 Aug 18;11(8): - PubMed
  39. Cells. 2019 Feb 01;8(2): - PubMed
  40. Nature. 2004 Feb 5;427(6974):504 - PubMed
  41. J Biosci Bioeng. 2012 Mar;113(3):381-8 - PubMed
  42. Biochem Genet. 2018 Aug;56(4):341-355 - PubMed
  43. Oncotarget. 2016 Oct 11;7(41):66606-66622 - PubMed
  44. Gene. 1993 Aug 25;130(2):305-6 - PubMed
  45. Biochim Biophys Acta Mol Cell Biol Lipids. 2019 Oct;1864(10):1375-1383 - PubMed
  46. Sci Rep. 2018 Feb 9;8(1):2781 - PubMed
  47. Sci Rep. 2015 Nov 13;5:16623 - PubMed

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