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Lei I, Huang W, Ward PA, et al. Differential inflammatory responses of the native left and right ventricle associated with donor heart preservation. Physiol Rep. 2021;9(17):e15004doi: 10.14814/phy2.15004.
Lei, I., Huang, W., Ward, P. A., Pober, J. S., Tellides, G., Ailawadi, G., Pagani, F. D., Landstrom, A. P., Wang, Z., Mortensen, R. M., Cascalho, M., Platt, J., Eugene Chen, Y., Lam, H. Y. K., & Tang, P. C. (2021). Differential inflammatory responses of the native left and right ventricle associated with donor heart preservation. Physiological reports, 9(17), e15004. https://doi.org/10.14814/phy2.15004
Lei, Ienglam, et al. "Differential inflammatory responses of the native left and right ventricle associated with donor heart preservation." Physiological reports vol. 9,17 (2021): e15004. doi: https://doi.org/10.14814/phy2.15004
Lei I, Huang W, Ward PA, Pober JS, Tellides G, Ailawadi G, Pagani FD, Landstrom AP, Wang Z, Mortensen RM, Cascalho M, Platt J, Eugene Chen Y, Lam HYK, Tang PC. Differential inflammatory responses of the native left and right ventricle associated with donor heart preservation. Physiol Rep. 2021 Sep;9(17):e15004. doi: 10.14814/phy2.15004. PMID: 34435466; PMCID: PMC8387788.
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Physiol Rep. 2021 Sep;9(17):e15004. doi: 10.14814/phy2.15004.

Differential inflammatory responses of the native left and right ventricle associated with donor heart preservation.

Physiological reports

Ienglam Lei, Wei Huang, Peter A Ward, Jordan S Pober, George Tellides, Gorav Ailawadi, Francis D Pagani, Andrew P Landstrom, Zhong Wang, Richard M Mortensen, Marilia Cascalho, Jeffrey Platt, Yuqing Eugene Chen, Hugo Yu Kor Lam, Paul C Tang

Affiliations

  1. Department of Cardiac Surgery, University of Michigan Frankel Cardiovascular Center, Ann Arbor, Michigan, USA.
  2. Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan, USA.
  3. Department of Immunobiology, Yale University, New Haven, Connecticut, USA.
  4. Department of Surgery, Yale University, New Haven, Connecticut, USA.
  5. Department of Pediatrics, Duke University, Durham, North Carolina, USA.
  6. Department of Internal Medicine, University of Michigan Frankel Cardiovascular Center, Ann Arbor, Michigan, USA.
  7. Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan, USA.
  8. Hypahub Inc, San Jose, California, USA.

PMID: 34435466 PMCID: PMC8387788 DOI: 10.14814/phy2.15004

Abstract

BACKGROUND: Dysfunction and inflammation of hearts subjected to cold ischemic preservation may differ between left and right ventricles, suggesting distinct strategies for amelioration.

METHODS AND RESULTS: Explanted murine hearts subjected to cold ischemia for 0, 4, or 8 h in preservation solution were assessed for function during 60 min of warm perfusion and then analyzed for cell death and inflammation by immunohistochemistry and western blotting and total RNA sequencing. Increased cold ischemic times led to greater left ventricle (LV) dysfunction compared to right ventricle (RV). The LV experienced greater cell death assessed by TUNEL

CONCLUSIONS: Mouse hearts subjected to cold ischemia showed time-dependent contractile dysfunction and increased cell death, inflammatory cytokine expression and inflammasome expression that are greater in the LV than RV. However, IL-6 protein elevations and altered transcriptional profiles were similar in both ventricles. Similar changes are observed in human hearts.

© 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.

Keywords: contractile function; inflammation; ischemia; myocardial biology; transplantation

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