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Zhang Q, Zou M, Wang W, et al. Design, Synthesis, and Characterization of Tracers and Development of a Fluorescence Polarization Immunoassay for Rapid Screening of 4,4'-Dinitrocarbanilide in Chicken Muscle. Foods. 2021;10(8)doi: 10.3390/foods10081822.
Zhang, Q., Zou, M., Wang, W., Li, J., & Liang, X. (2021). Design, Synthesis, and Characterization of Tracers and Development of a Fluorescence Polarization Immunoassay for Rapid Screening of 4,4'-Dinitrocarbanilide in Chicken Muscle. Foods (Basel, Switzerland), 10(8), . https://doi.org/10.3390/foods10081822
Zhang, Qidi, et al. "Design, Synthesis, and Characterization of Tracers and Development of a Fluorescence Polarization Immunoassay for Rapid Screening of 4,4'-Dinitrocarbanilide in Chicken Muscle." Foods (Basel, Switzerland) vol. 10,8 (2021). doi: https://doi.org/10.3390/foods10081822
Zhang Q, Zou M, Wang W, Li J, Liang X. Design, Synthesis, and Characterization of Tracers and Development of a Fluorescence Polarization Immunoassay for Rapid Screening of 4,4'-Dinitrocarbanilide in Chicken Muscle. Foods. 2021 Aug 06;10(8). doi: 10.3390/foods10081822. PMID: 34441602; PMCID: PMC8392466.
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Foods. 2021 Aug 06;10(8). doi: 10.3390/foods10081822.

Design, Synthesis, and Characterization of Tracers and Development of a Fluorescence Polarization Immunoassay for Rapid Screening of 4,4'-Dinitrocarbanilide in Chicken Muscle.

Foods (Basel, Switzerland)

Qidi Zhang, Ming Zou, Wanyu Wang, Jinyan Li, Xiao Liang

Affiliations

  1. College of Veterinary Medicine, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, China.
  2. Basic Medical College, Qingdao University, No. 308 Ningxia Road, Qingdao 266071, China.

PMID: 34441602 PMCID: PMC8392466 DOI: 10.3390/foods10081822

Abstract

The compound, 4,4'-dinitrocarbanilide (DNC), is the marker residue of concern in edible tissues of broilers fed with diets containing anticoccidial nicarbazin (NIC). In this study, 25 fluorescein-labeled DNC derivatives (tracers) are synthesized and characterized to develop a rapid fluorescence polarization immunoassay (FPIA) for the detection of DNC in chickens using DNC monoclonal antibodies (mAbs). The effect of the tracer structure on the sensitivity of the FPIA is investigated. Our results show that after optimization, the half maximal inhibitory concentrations (IC

Keywords: DNC residue; FPIA; chicken muscle; tracers

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