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Gross M, Ashqar F, Sionov RV, et al. Sustained release varnish containing chlorhexidine for prevention of Streptococcus mutans biofilm formation on voice prosthesis surface: an in vitro study. Int Microbiol. 2021;doi: 10.1007/s10123-021-00205-w.
Gross, M., Ashqar, F., Sionov, R. V., Friedman, M., Eliashar, R., Zaks, B., Gati, I., Duanis-Assaf, D., Feldman, M., & Steinberg, D. (2021). Sustained release varnish containing chlorhexidine for prevention of Streptococcus mutans biofilm formation on voice prosthesis surface: an in vitro study. International microbiology : the official journal of the Spanish Society for Microbiology, . https://doi.org/10.1007/s10123-021-00205-w
Gross, Menachem, et al. "Sustained release varnish containing chlorhexidine for prevention of Streptococcus mutans biofilm formation on voice prosthesis surface: an in vitro study." International microbiology : the official journal of the Spanish Society for Microbiology vol. (2021). doi: https://doi.org/10.1007/s10123-021-00205-w
Gross M, Ashqar F, Sionov RV, Friedman M, Eliashar R, Zaks B, Gati I, Duanis-Assaf D, Feldman M, Steinberg D. Sustained release varnish containing chlorhexidine for prevention of Streptococcus mutans biofilm formation on voice prosthesis surface: an in vitro study. Int Microbiol. 2021 Sep 09; doi: 10.1007/s10123-021-00205-w. Epub 2021 Sep 09. PMID: 34505216.
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Int Microbiol. 2021 Sep 09; doi: 10.1007/s10123-021-00205-w. Epub 2021 Sep 09.

Sustained release varnish containing chlorhexidine for prevention of Streptococcus mutans biofilm formation on voice prosthesis surface: an in vitro study.

International microbiology : the official journal of the Spanish Society for Microbiology

Menachem Gross, Fadi Ashqar, Ronit Vogt Sionov, Michael Friedman, Ron Eliashar, Batya Zaks, Irith Gati, Danielle Duanis-Assaf, Mark Feldman, Doron Steinberg

Affiliations

  1. Department of Otolaryngology - Head and Neck Surgery, Hadassah Hebrew University Medical Center, P.O.Box 12000, 91120, Jerusalem, Israel. [email protected].
  2. Biofilm Research Laboratory, Institute of Dental Sciences, Hebrew University, 91120, Jerusalem, Israel. [email protected].
  3. School of Medicine, Hebrew University, Jerusalem, Israel. [email protected].
  4. Department of Otolaryngology - Head and Neck Surgery, Hadassah Hebrew University Medical Center, P.O.Box 12000, 91120, Jerusalem, Israel.
  5. Biofilm Research Laboratory, Institute of Dental Sciences, Hebrew University, 91120, Jerusalem, Israel.
  6. School of Medicine, Hebrew University, Jerusalem, Israel.
  7. School of Pharmacy, Hebrew University, Jerusalem, Israel.

PMID: 34505216 DOI: 10.1007/s10123-021-00205-w

Abstract

OBJECTIVES: In this study, we aimed to develop a novel, sustained release varnish (SRV) for voice prostheses (VP) releasing chlorhexidine (CHX), for the prevention of biofilm formation caused by the common oral bacteria Streptococcus mutans on VP surfaces.

METHODS: This study was performed in an in vitro model as a step towards future in vivo trials. VPs were coated with a SRV containing CHX (SRV-CHX) or SRV alone (placebo-SRV) that were daily exposed to S. mutans. The polymeric materials of SRV were composed of ethylcellulose and PEG-400. Biofilm formation was assessed by DNA quantification (qPCR), crystal violet staining, confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM), and kinetics experiments.

RESULTS: The amount of DNA in the biofilms formed by S. mutans on VP surfaces coated once with SRV-CHX (1.024 ± 0.218 ng DNA/piece) was 58.5 ± 8.8% lower than that of placebo-SRV-coated VPs (2.465 ± 0.198 ng DNA/piece) after a 48-h exposure to S. mutans (p = 0.038). Reduced biofilm mass on SRV-CHX-coated VPs was visually confirmed by CLSM and SEM. CV staining of SRV-CHX single-coated VPs that have been exposed to S. mutans nine times showed a 98.1 ± 0.2% reduction in biofilm mass compared to placebo-SRV-coated VPs (p = 0.003). Kinetic experiments revealed that SRV-CHX triple-coated VPs could delay bacterial growth for 23 days.

CONCLUSIONS: Coating VPs with SRV-CHX has an inhibitory effect on biofilm formation and prevents bacterial growth in their vicinities. This study is a proof-of-principle that paves the way for developing new clinical means for reducing both VPs' bacterial biofilm formation and device failure.

© 2021. The Author(s), under exclusive licence to Springer Nature Switzerland AG.

Keywords: Biofilm; Chlorhexidine; Laryngectomy; Sustained release varnish; Voice prosthesis

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