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Wang Y, Sun J, Guo X, et al. A SYBR Green I-based real-time polymerase chain reaction assay for detection and quantification of canine bufavirus. Mol Cell Probes. 2021;59:101762doi: 10.1016/j.mcp.2021.101762.
Wang, Y., Sun, J., Guo, X., Li, W., Zhang, D., Liu, G., Zhou, T., & Li, Y. (2021). A SYBR Green I-based real-time polymerase chain reaction assay for detection and quantification of canine bufavirus. Molecular and cellular probes, 59101762. https://doi.org/10.1016/j.mcp.2021.101762
Wang, Yong, et al. "A SYBR Green I-based real-time polymerase chain reaction assay for detection and quantification of canine bufavirus." Molecular and cellular probes vol. 59 (2021): 101762. doi: https://doi.org/10.1016/j.mcp.2021.101762
Wang Y, Sun J, Guo X, Li W, Zhang D, Liu G, Zhou T, Li Y. A SYBR Green I-based real-time polymerase chain reaction assay for detection and quantification of canine bufavirus. Mol Cell Probes. 2021 Oct;59:101762. doi: 10.1016/j.mcp.2021.101762. Epub 2021 Sep 02. PMID: 34481896.
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Mol Cell Probes. 2021 Oct;59:101762. doi: 10.1016/j.mcp.2021.101762. Epub 2021 Sep 02.

A SYBR Green I-based real-time polymerase chain reaction assay for detection and quantification of canine bufavirus.

Molecular and cellular probes

Yong Wang, Jianfei Sun, Xu Guo, Wei Li, Da Zhang, Guangqing Liu, Tianhong Zhou, Yongdong Li

Affiliations

  1. Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China.
  2. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, PR China.
  3. Municipal Key Laboratory of Virology, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, PR China. Electronic address: [email protected].

PMID: 34481896 DOI: 10.1016/j.mcp.2021.101762

Abstract

Canine bufavirus (CBuV) was first discovered in puppies in Italy in 2016, and subsequent studies have reported its possible relationship with acute enteritis. Currently, there is no specific and quantitative detection method for CBuV. This study examined the conserved NS1 gene and used a pair of specific primers to establish a direct SYBR Green I-based real-time quantitative polymerase chain reaction (qPCR) method for the detection and quantification of CBuV. In the sensitivity experiment, the detection limit of SYBR Green I-based real-time qPCR was 4.676 × 10

Copyright © 2021 Elsevier Ltd. All rights reserved.

Keywords: Canine bufavirus; Quantitative analysis; Real-time polymerase chain reaction; SYBR Green I

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