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Amler AK, Schlauch D, Tüzüner S, et al. Pilot investigation on the dose-dependent impact of irradiation on primary human alveolar osteoblasts in vitro. Sci Rep. 2021;11(1):19833doi: 10.1038/s41598-021-99323-8.
Amler, A. K., Schlauch, D., Tüzüner, S., Thomas, A., Neckel, N., Tinhofer, I., Heiland, M., Lauster, R., Kloke, L., Stromberger, C., & Nahles, S. (2021). Pilot investigation on the dose-dependent impact of irradiation on primary human alveolar osteoblasts in vitro. Scientific reports, 11(1), 19833. https://doi.org/10.1038/s41598-021-99323-8
Amler, Anna-Klara, et al. "Pilot investigation on the dose-dependent impact of irradiation on primary human alveolar osteoblasts in vitro." Scientific reports vol. 11,1 (2021): 19833. doi: https://doi.org/10.1038/s41598-021-99323-8
Amler AK, Schlauch D, Tüzüner S, Thomas A, Neckel N, Tinhofer I, Heiland M, Lauster R, Kloke L, Stromberger C, Nahles S. Pilot investigation on the dose-dependent impact of irradiation on primary human alveolar osteoblasts in vitro. Sci Rep. 2021 Oct 06;11(1):19833. doi: 10.1038/s41598-021-99323-8. PMID: 34615948; PMCID: PMC8494843.
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Sci Rep. 2021 Oct 06;11(1):19833. doi: 10.1038/s41598-021-99323-8.

Pilot investigation on the dose-dependent impact of irradiation on primary human alveolar osteoblasts in vitro.

Scientific reports

Anna-Klara Amler, Domenic Schlauch, Selin Tüzüner, Alexander Thomas, Norbert Neckel, Ingeborg Tinhofer, Max Heiland, Roland Lauster, Lutz Kloke, Carmen Stromberger, Susanne Nahles

Affiliations

  1. Cellbricks GmbH, Berlin, Germany. [email protected].
  2. Department of Medical Biotechnology, Technische Universität Berlin, Berlin, Germany. [email protected].
  3. Cellbricks GmbH, Berlin, Germany.
  4. Department of Medical Biotechnology, Technische Universität Berlin, Berlin, Germany.
  5. Department of Oral and Maxillofacial Surgery, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt Universität zu Berlin, Berlin, Germany.
  6. Department of Radiation Oncology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin, Germany.
  7. German Cancer Consortium (DKTK) Partner Site Berlin, Berlin, Germany.

PMID: 34615948 PMCID: PMC8494843 DOI: 10.1038/s41598-021-99323-8

Abstract

Radiotherapy of head and neck squamous cell carcinoma can lead to long-term complications like osteoradionecrosis, resulting in severe impairment of the jawbone. Current standard procedures require a 6-month wait after irradiation before dental reconstruction can begin. A comprehensive characterization of the irradiation-induced molecular and functional changes in bone cells could allow the development of novel strategies for an earlier successful dental reconstruction in patients treated by radiotherapy. The impact of ionizing radiation on the bone-forming alveolar osteoblasts remains however elusive, as previous studies have relied on animal-based models and fetal or animal-derived cell lines. This study presents the first in vitro data obtained from primary human alveolar osteoblasts. Primary human alveolar osteoblasts were isolated from healthy donors and expanded. After X-ray irradiation with 2, 6 and 10 Gy, cells were cultivated under osteogenic conditions and analyzed regarding their proliferation, mineralization, and expression of marker genes and proteins. Proliferation of osteoblasts decreased in a dose-dependent manner. While cells recovered from irradiation with 2 Gy, application of 6 and 10 Gy doses not only led to a permanent impairment of proliferation, but also resulted in altered cell morphology and a disturbed structure of the extracellular matrix as demonstrated by immunostaining of collagen I and fibronectin. Following irradiation with any of the examined doses, a decrease of marker gene expression levels was observed for most of the investigated genes, revealing interindividual differences. Primary human alveolar osteoblasts presented a considerably changed phenotype after irradiation, depending on the dose administered. Mechanisms for these findings need to be further investigated. This could facilitate improved patient care by re-evaluating current standard procedures and investigating faster and safer reconstruction concepts, thus improving quality of life and social integrity.

© 2021. The Author(s).

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