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Wilson RL, Lampe K, Matushewski BJ, et al. Time Mating Guinea Pigs by Monitoring Changes to the Vaginal Membrane throughout the Estrus Cycle and with Ultrasound Confirmation. Methods Protoc. 2021;4(3)doi: 10.3390/mps4030058.
Wilson, R. L., Lampe, K., Matushewski, B. J., Regnault, T. R. H., & Jones, H. N. (2021). Time Mating Guinea Pigs by Monitoring Changes to the Vaginal Membrane throughout the Estrus Cycle and with Ultrasound Confirmation. Methods and protocols, 4(3), . https://doi.org/10.3390/mps4030058
Wilson, Rebecca L, et al. "Time Mating Guinea Pigs by Monitoring Changes to the Vaginal Membrane throughout the Estrus Cycle and with Ultrasound Confirmation." Methods and protocols vol. 4,3 (2021). doi: https://doi.org/10.3390/mps4030058
Wilson RL, Lampe K, Matushewski BJ, Regnault TRH, Jones HN. Time Mating Guinea Pigs by Monitoring Changes to the Vaginal Membrane throughout the Estrus Cycle and with Ultrasound Confirmation. Methods Protoc. 2021 Aug 27;4(3). doi: 10.3390/mps4030058. PMID: 34564304; PMCID: PMC8482275.
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Methods Protoc. 2021 Aug 27;4(3). doi: 10.3390/mps4030058.

Time Mating Guinea Pigs by Monitoring Changes to the Vaginal Membrane throughout the Estrus Cycle and with Ultrasound Confirmation.

Methods and protocols

Rebecca L Wilson, Kristin Lampe, Brad J Matushewski, Timothy R H Regnault, Helen N Jones

Affiliations

  1. Center for Research in Perinatal Outcomes, College of Medicine, University of Florida, Gainesville, FL 32610, USA.
  2. Department of Physiology and Functional Genomics, College of Medicine, University of Florida, Gainesville, FL 32610, USA.
  3. Center for Fetal and Placental Research, Cincinnati Children's Hospital and Medical Center, Cincinnati, OH 45229, USA.
  4. Department of Kinesiology, Western University, London, ON N6A 5B9, Canada.
  5. Departments of Physiology and Pharmacology, and Obstetrics and Gynaecology, Schulich School of Medicine and Dentistry, Western University, London, ON N6A 5B9, Canada.
  6. Children's Health Research Institute and Lawson Health Research Institute, London, ON N6A 5B9, Canada.

PMID: 34564304 PMCID: PMC8482275 DOI: 10.3390/mps4030058

Abstract

One of the greatest challenges to the development and implementation of pregnancy therapeutics is the ability to rigorously test treatments in clinically relevant animal models. Guinea pigs offer a unique advantage in studying the placenta, fetal development, and reproductive health as they have similar developmental milestones to humans, both throughout gestation and following birth. Tracking the guinea pig estrus cycle is imperative to ensuring appropriately timed mating and can be performed by monitoring the guinea pig vaginal membrane. Here, we describe a methodology to efficiently and accurately time mate guinea pigs, and provide a picture representation of changes to the guinea pig vaginal membrane throughout the estrus cycle. Utilization of this monitoring enabled a 100% pregnancy success rate on the first mating attempt in a cohort of five guinea pigs. This approach, along with early pregnancy ultrasounds as a secondary method to confirm pregnancy, offers a reliable approach to timed mating in the guinea pig.

Keywords: guinea pig; methodology; pregnancy; reproduction; time mate

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