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Hermes L, Römermann J, Cramer B, et al. Phase II Metabolism of Asarone Isomers In Vitro and in Humans Using HPLC-MS/MS and HPLC-qToF/MS. Foods. 2021;10(9)doi: 10.3390/foods10092032.
Hermes, L., Römermann, J., Cramer, B., & Esselen, M. (2021). Phase II Metabolism of Asarone Isomers In Vitro and in Humans Using HPLC-MS/MS and HPLC-qToF/MS. Foods (Basel, Switzerland), 10(9), . https://doi.org/10.3390/foods10092032
Hermes, Lena, et al. "Phase II Metabolism of Asarone Isomers In Vitro and in Humans Using HPLC-MS/MS and HPLC-qToF/MS." Foods (Basel, Switzerland) vol. 10,9 (2021). doi: https://doi.org/10.3390/foods10092032
Hermes L, Römermann J, Cramer B, Esselen M. Phase II Metabolism of Asarone Isomers In Vitro and in Humans Using HPLC-MS/MS and HPLC-qToF/MS. Foods. 2021 Aug 29;10(9). doi: 10.3390/foods10092032. PMID: 34574142; PMCID: PMC8467817.
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Foods. 2021 Aug 29;10(9). doi: 10.3390/foods10092032.

Phase II Metabolism of Asarone Isomers In Vitro and in Humans Using HPLC-MS/MS and HPLC-qToF/MS.

Foods (Basel, Switzerland)

Lena Hermes, Janis Römermann, Benedikt Cramer, Melanie Esselen

Affiliations

  1. Institute of Food Chemistry, University of Muenster, Corrensstraße 45, 48149 Muenster, Germany.

PMID: 34574142 PMCID: PMC8467817 DOI: 10.3390/foods10092032

Abstract

(1) Background: Metabolism data of asarone isomers, in particular phase II, in vitro and in humans is limited so far. For the first time, phase II metabolites of asarone isomers were characterized and human kinetic as well as excretion data after oral intake of asarone-containing tea infusion was determined. (2) Methods: A high pressure liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-qTOF-MS) approach was used to identify phase II metabolites using liver microsomes of different species and in human urine samples. For quantitation of the respective glucuronides, a beta-glucuronidase treatment was performed prior to analysis via high pressure liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). (3) Results: Ingested beta-asarone and

Keywords: asarone glucuronides; asarone isomers; human study; metabolites

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