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Patel A, Walters JN, Reuschel EL, et al. Intradermal-delivered DNA vaccine induces durable immunity mediating a reduction in viral load in a rhesus macaque SARS-CoV-2 challenge model. Cell Rep Med. 2021;2(10):100420doi: 10.1016/j.xcrm.2021.100420.
Patel, A., Walters, J. N., Reuschel, E. L., Schultheis, K., Parzych, E., Gary, E. N., Maricic, I., Purwar, M., Eblimit, Z., Walker, S. N., Guimet, D., Bhojnagarwala, P., Adeniji, O. S., Doan, A., Xu, Z., Elwood, D., Reeder, S. M., Pessaint, L., Kim, K. Y., Cook, A., Chokkalingam, N., Finneyfrock, B., Tello-Ruiz, E., Dodson, A., Choi, J., Generotti, A., Harrison, J., Tursi, N. J., Andrade, V. M., Dia, Y., Zaidi, F. I., Andersen, H., Abdel-Mohsen, M., Lewis, M. G., Muthumani, K., Kim, J. J., Kulp, D. W., Humeau, L. M., Ramos, S. J., Smith, T. R. F., Weiner, D. B., & Broderick, K. E. (2021). Intradermal-delivered DNA vaccine induces durable immunity mediating a reduction in viral load in a rhesus macaque SARS-CoV-2 challenge model. Cell reports. Medicine, 2(10), 100420. https://doi.org/10.1016/j.xcrm.2021.100420
Patel, Ami, et al. "Intradermal-delivered DNA vaccine induces durable immunity mediating a reduction in viral load in a rhesus macaque SARS-CoV-2 challenge model." Cell reports. Medicine vol. 2,10 (2021): 100420. doi: https://doi.org/10.1016/j.xcrm.2021.100420
Patel A, Walters JN, Reuschel EL, Schultheis K, Parzych E, Gary EN, Maricic I, Purwar M, Eblimit Z, Walker SN, Guimet D, Bhojnagarwala P, Adeniji OS, Doan A, Xu Z, Elwood D, Reeder SM, Pessaint L, Kim KY, Cook A, Chokkalingam N, Finneyfrock B, Tello-Ruiz E, Dodson A, Choi J, Generotti A, Harrison J, Tursi NJ, Andrade VM, Dia Y, Zaidi FI, Andersen H, Abdel-Mohsen M, Lewis MG, Muthumani K, Kim JJ, Kulp DW, Humeau LM, Ramos SJ, Smith TRF, Weiner DB, Broderick KE. Intradermal-delivered DNA vaccine induces durable immunity mediating a reduction in viral load in a rhesus macaque SARS-CoV-2 challenge model. Cell Rep Med. 2021 Oct 19;2(10):100420. doi: 10.1016/j.xcrm.2021.100420. Epub 2021 Sep 28. PMID: 34604818; PMCID: PMC8479327.
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Cell Rep Med. 2021 Oct 19;2(10):100420. doi: 10.1016/j.xcrm.2021.100420. Epub 2021 Sep 28.

Intradermal-delivered DNA vaccine induces durable immunity mediating a reduction in viral load in a rhesus macaque SARS-CoV-2 challenge model.

Cell reports. Medicine

Ami Patel, Jewell N Walters, Emma L Reuschel, Katherine Schultheis, Elizabeth Parzych, Ebony N Gary, Igor Maricic, Mansi Purwar, Zeena Eblimit, Susanne N Walker, Diana Guimet, Pratik Bhojnagarwala, Opeyemi S Adeniji, Arthur Doan, Ziyang Xu, Dustin Elwood, Sophia M Reeder, Laurent Pessaint, Kevin Y Kim, Anthony Cook, Neethu Chokkalingam, Brad Finneyfrock, Edgar Tello-Ruiz, Alan Dodson, Jihae Choi, Alison Generotti, John Harrison, Nicholas J Tursi, Viviane M Andrade, Yaya Dia, Faraz I Zaidi, Hanne Andersen, Mohamed Abdel-Mohsen, Mark G Lewis, Kar Muthumani, J Joseph Kim, Daniel W Kulp, Laurent M Humeau, Stephanie J Ramos, Trevor R F Smith, David B Weiner, Kate E Broderick

Affiliations

  1. Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  2. Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA.
  3. Bioqual, Rockville, MD 20852, USA.

PMID: 34604818 PMCID: PMC8479327 DOI: 10.1016/j.xcrm.2021.100420

Abstract

Coronavirus disease 2019 (COVID-19), caused by the SARS-CoV-2 virus, has had a dramatic global impact on public health and social and economic infrastructures. Here, we assess the immunogenicity and anamnestic protective efficacy in rhesus macaques of an intradermal (i.d.)-delivered SARS-CoV-2 spike DNA vaccine, INO-4800, currently being evaluated in clinical trials. Vaccination with INO-4800 induced T cell responses and induced spike antigen and RBD binding antibodies with ADCP and ADCD activity. Sera from the animals neutralized both the D614 and G614 SARS-CoV-2 pseudotype viruses. Several months after vaccination, animals were challenged with SARS-CoV-2 resulting in rapid recall of anti-SARS-CoV-2 spike protein T cell and neutralizing antibody responses. These responses were associated with lower viral loads in the lung. These studies support the immune impact of INO-4800 for inducing both humoral and cellular arms of the adaptive immune system, which are likely important for providing durable protection against COVID-19 disease.

© 2021 The Author(s).

Keywords: COVID-19; ID DNA vaccine; SARS-CoV-2; challenge; coronavirus; electroporation; infectious disease; macaque; protection

Conflict of interest statement

A.P., E.L.R., E.P., E.N.G., M.P., S.N.W., P.B., Z.X., S.M.R., K.Y.K., N.C., E.T-R., J.C., N.J.T., K.M., and D.K.W. declare no competing interests. J.N.W., K.S., I.M., Z.E., D.G., A.D., D.E., A.G., V.M

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