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Wang P, Casner RG, Nair MS, et al. A monoclonal antibody that neutralizes SARS-CoV-2 variants, SARS-CoV, and other sarbecoviruses. bioRxiv. 2021;doi: 10.1101/2021.10.13.464307.
Wang, P., Casner, R. G., Nair, M. S., Yu, J., Guo, Y., Wang, M., Chan, J. F., Cerutti, G., Iketani, S., Liu, L., Sheng, Z., Chen, Z., Yuen, K. Y., Kwong, P. D., Huang, Y., Shapiro, L., & Ho, D. D. (2021). A monoclonal antibody that neutralizes SARS-CoV-2 variants, SARS-CoV, and other sarbecoviruses. bioRxiv : the preprint server for biology, . https://doi.org/10.1101/2021.10.13.464307
Wang, Pengfei, et al. "A monoclonal antibody that neutralizes SARS-CoV-2 variants, SARS-CoV, and other sarbecoviruses." bioRxiv : the preprint server for biology vol. (2021). doi: https://doi.org/10.1101/2021.10.13.464307
Wang P, Casner RG, Nair MS, Yu J, Guo Y, Wang M, Chan JF, Cerutti G, Iketani S, Liu L, Sheng Z, Chen Z, Yuen KY, Kwong PD, Huang Y, Shapiro L, Ho DD. A monoclonal antibody that neutralizes SARS-CoV-2 variants, SARS-CoV, and other sarbecoviruses. bioRxiv. 2021 Oct 14; doi: 10.1101/2021.10.13.464307. PMID: 34671769; PMCID: PMC8528075.
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bioRxiv. 2021 Oct 14; doi: 10.1101/2021.10.13.464307.

A monoclonal antibody that neutralizes SARS-CoV-2 variants, SARS-CoV, and other sarbecoviruses.

bioRxiv : the preprint server for biology

Pengfei Wang, Ryan G Casner, Manoj S Nair, Jian Yu, Yicheng Guo, Maple Wang, Jasper F-W Chan, Gabriele Cerutti, Sho Iketani, Lihong Liu, Zizhang Sheng, Zhiwei Chen, Kwok-Yung Yuen, Peter D Kwong, Yaoxing Huang, Lawrence Shapiro, David D Ho

Affiliations

  1. Aaron Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA.
  2. Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
  3. State Key Laboratory of Emerging Infectious Diseases, Carol Yu Centre for Infection, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.
  4. Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science and Technology Park, Hong Kong Special Administrative Region, China.
  5. Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA.
  6. Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY 10032, USA.
  7. Division of Infectious Diseases, Department of Internal Medicine, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA.

PMID: 34671769 PMCID: PMC8528075 DOI: 10.1101/2021.10.13.464307

Abstract

The repeated emergence of highly pathogenic human coronaviruses as well as their evolving variants highlight the need to develop potent and broad-spectrum antiviral therapeutics and vaccines. By screening monoclonal antibodies (mAbs) isolated from COVID-19-convalescent patients, we found one mAb, 2-36, with cross-neutralizing activity against SARS-CoV. We solved the cryo-EM structure of 2-36 in complex with SARS-CoV-2 or SARS-CoV spike, revealing a highly conserved epitope in the receptor-binding domain (RBD). Antibody 2-36 neutralized not only all current circulating SARS-CoV-2 variants and SARS-COV, but also a panel of bat and pangolin sarbecoviruses that can use human angiotensin-converting enzyme 2 (ACE2) as a receptor. We selected 2-36-escape viruses

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