Display options
Cite
Tsou AM, Goettel JA, Bao B, et al. Utilizing a reductionist model to study host-microbe interactions in intestinal inflammation. Microbiome. 2021;9(1):215doi: 10.1186/s40168-021-01161-3.
Tsou, A. M., Goettel, J. A., Bao, B., Biswas, A., Kang, Y. H., Redhu, N. S., Peng, K., Putzel, G. G., Saltzman, J., Kelly, R., Gringauz, J., Barends, J., Hatazaki, M., Frei, S. M., Emani, R., Huang, Y., Shen, Z., Fox, J. G., Glickman, J. N., Horwitz, B. H., & Snapper, S. B. (2021). Utilizing a reductionist model to study host-microbe interactions in intestinal inflammation. Microbiome, 9(1), 215. https://doi.org/10.1186/s40168-021-01161-3
Tsou, Amy M, et al. "Utilizing a reductionist model to study host-microbe interactions in intestinal inflammation." Microbiome vol. 9,1 (2021): 215. doi: https://doi.org/10.1186/s40168-021-01161-3
Tsou AM, Goettel JA, Bao B, Biswas A, Kang YH, Redhu NS, Peng K, Putzel GG, Saltzman J, Kelly R, Gringauz J, Barends J, Hatazaki M, Frei SM, Emani R, Huang Y, Shen Z, Fox JG, Glickman JN, Horwitz BH, Snapper SB. Utilizing a reductionist model to study host-microbe interactions in intestinal inflammation. Microbiome. 2021 Nov 03;9(1):215. doi: 10.1186/s40168-021-01161-3. PMID: 34732258; PMCID: PMC8565002.
Copy Download .nbib Format: NLM
Share it on

Microbiome. 2021 Nov 03;9(1):215. doi: 10.1186/s40168-021-01161-3.

Utilizing a reductionist model to study host-microbe interactions in intestinal inflammation.

Microbiome

Amy M Tsou, Jeremy A Goettel, Bin Bao, Amlan Biswas, Yu Hui Kang, Naresh S Redhu, Kaiyue Peng, Gregory G Putzel, Jeffrey Saltzman, Ryan Kelly, Jordan Gringauz, Jared Barends, Mai Hatazaki, Sandra M Frei, Rohini Emani, Ying Huang, Zeli Shen, James G Fox, Jonathan N Glickman, Bruce H Horwitz, Scott B Snapper

Affiliations

  1. Division of Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Boston, MA, USA. [email protected].
  2. Harvard Medical School, Boston, MA, USA. [email protected].
  3. Jill Roberts Institute for Research in Inflammatory Bowel Disease, Weill Cornell Medical College, New York, NY, USA. [email protected].
  4. Division of Pediatric Gastroenterology and Nutrition, Weill Cornell Medical College, New York, NY, USA. [email protected].
  5. Division of Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Boston, MA, USA.
  6. Harvard Medical School, Boston, MA, USA.
  7. Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.
  8. Department of Gastroenterology, Children's Hospital of Fudan University, Shanghai, China.
  9. Jill Roberts Institute for Research in Inflammatory Bowel Disease, Weill Cornell Medical College, New York, NY, USA.
  10. Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA.
  11. Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA, USA.
  12. Division of Emergency Medicine, Boston Children's Hospital, Boston, MA, USA.
  13. Division of Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Boston, MA, USA. [email protected].
  14. Harvard Medical School, Boston, MA, USA. [email protected].
  15. Division of Gastroenterology, Brigham and Women's Hospital, Boston, MA, USA. [email protected].

PMID: 34732258 PMCID: PMC8565002 DOI: 10.1186/s40168-021-01161-3

Abstract

BACKGROUND: The gut microbiome is altered in patients with inflammatory bowel disease, yet how these alterations contribute to intestinal inflammation is poorly understood. Murine models have demonstrated the importance of the microbiome in colitis since colitis fails to develop in many genetically susceptible animal models when re-derived into germ-free environments. We have previously shown that Wiskott-Aldrich syndrome protein (WASP)-deficient mice (Was

RESULTS: Was

CONCLUSIONS: These studies indicate that the effect of a microbe on the immune system can be context dependent, with the same bacteria eliciting a tolerogenic response under homeostatic conditions but promoting inflammation in immune-dysregulated hosts. Furthermore, in inflamed environments, some bacteria up-regulate genes that enhance their fitness and immunogenicity, while other bacteria are less able to adapt and decrease in abundance. These findings highlight the importance of studying host-microbe interactions in different contexts and considering how the transcriptional profile and fitness of bacteria may change in different hosts when developing microbiota-based therapeutics. Video abstract.

© 2021. The Author(s).

Keywords: Defined consortium; Gut microbiota; Immune dysregulation; Intestinal inflammation; Pathobiont; Wiskott-Aldrich syndrome

References

  1. Nature. 2012 Nov 1;491(7422):119-24 - PubMed
  2. Sci Immunol. 2019 Apr 19;4(34): - PubMed
  3. Sci Immunol. 2017 Jul 21;2(13): - PubMed
  4. Cochrane Database Syst Rev. 2018 Nov 13;11:CD012774 - PubMed
  5. Nature. 2015 Mar 5;519(7541):92-6 - PubMed
  6. Nat Immunol. 2011 Apr;12(4):320-6 - PubMed
  7. Proc Natl Acad Sci U S A. 2019 Dec 3;116(49):24760-24769 - PubMed
  8. Gut. 2016 Feb;65(2):225-37 - PubMed
  9. Nature. 2011 Sep 14;477(7366):596-600 - PubMed
  10. J Biol Chem. 1999 Oct 1;274(40):28083-6 - PubMed
  11. PLoS Pathog. 2020 Apr 10;16(4):e1008466 - PubMed
  12. Gut. 2014 Feb;63(2):281-91 - PubMed
  13. Cell. 2007 Oct 5;131(1):33-45 - PubMed
  14. Nat Genet. 2015 Sep;47(9):979-986 - PubMed
  15. Pediatrics. 2003 May;111(5 Pt 1):e622-7 - PubMed
  16. Nat Methods. 2016 Jul;13(7):581-3 - PubMed
  17. PLoS One. 2013 Apr 22;8(4):e61217 - PubMed
  18. J Clin Invest. 2008 Feb;118(2):534-44 - PubMed
  19. Infect Immun. 2011 Sep;79(9):3711-7 - PubMed
  20. Gut. 2014 Oct;63(10):1566-77 - PubMed
  21. Inflamm Bowel Dis. 2013 Sep;19(10):2041-50 - PubMed
  22. Am J Gastroenterol. 2011 Apr;106(4):661-73 - PubMed
  23. Genome Biol. 2014;15(12):550 - PubMed
  24. Science. 2013 Aug 2;341(6145):569-73 - PubMed
  25. Nature. 2013 Dec 19;504(7480):451-5 - PubMed
  26. Cell Host Microbe. 2017 Feb 8;21(2):208-219 - PubMed
  27. Gastroenterology. 2007 Oct;133(4):1188-97 - PubMed
  28. ISME J. 2012 Nov;6(11):2091-106 - PubMed
  29. Bioinformatics. 2010 Oct 1;26(19):2460-1 - PubMed
  30. Vaccines (Basel). 2020 Dec 17;8(4): - PubMed
  31. Front Immunol. 2020 Feb 18;11:214 - PubMed
  32. Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1199-1204 - PubMed
  33. Gut. 2019 Feb;68(2):248-262 - PubMed
  34. Nat Commun. 2015 Sep 22;6:8292 - PubMed
  35. Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6 - PubMed
  36. Gut. 2014 Jul;63(7):1069-80 - PubMed
  37. Immunity. 1998 Jul;9(1):81-91 - PubMed
  38. J Clin Invest. 2004 May;113(9):1296-306 - PubMed
  39. ISME J. 2017 Feb;11(2):426-438 - PubMed
  40. Nucleic Acids Res. 2019 May 7;47(8):e47 - PubMed
  41. ILAR J. 2015;56(2):169-78 - PubMed
  42. Genes Dev. 1992 Dec;6(12B):2646-54 - PubMed
  43. PLoS Pathog. 2016 Jan 07;12(1):e1005295 - PubMed
  44. Nature. 2008 May 29;453(7195):620-5 - PubMed
  45. Lancet. 1991 Sep 28;338(8770):771-4 - PubMed
  46. Nat Methods. 2012 Mar 04;9(4):357-9 - PubMed
  47. J Crohns Colitis. 2017 Oct 1;11(10):1180-1199 - PubMed
  48. J Bacteriol. 2015 Oct;197(19):3206-15 - PubMed
  49. Nature. 2018 Feb 15;554(7692):373-377 - PubMed
  50. Appl Environ Microbiol. 2002 Jul;68(7):3401-7 - PubMed
  51. Cell. 1994 Aug 26;78(4):635-44 - PubMed
  52. Nucleic Acids Res. 2014 Jan;42(Database issue):D633-42 - PubMed
  53. Sci Rep. 2017 Dec 13;7(1):17522 - PubMed
  54. J Bacteriol. 1992 Oct;174(19):6256-63 - PubMed
  55. J Exp Med. 2013 Jun 3;210(6):1117-24 - PubMed
  56. Nat Med. 2019 Jul;25(7):1104-1109 - PubMed
  57. Nature. 2018 Jan 11;553(7687):208-211 - PubMed
  58. Gastroenterology. 1998 Feb;114(2):262-7 - PubMed
  59. Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13732-7 - PubMed
  60. Appl Environ Microbiol. 1999 Aug;65(8):3287-92 - PubMed
  61. Nat Methods. 2018 Nov;15(11):962-968 - PubMed
  62. Infect Immun. 2004 Mar;72(3):1364-73 - PubMed
  63. Annu Rev Immunol. 2002;20:495-549 - PubMed
  64. Nat Rev Gastroenterol Hepatol. 2021 Jul;18(7):503-513 - PubMed
  65. Cell Host Microbe. 2013 Nov 13;14(5):571-81 - PubMed
  66. J Exp Med. 1965 Jul 1;122:77-82 - PubMed
  67. Nat Genet. 2017 Feb;49(2):256-261 - PubMed
  68. Gut. 2002 Feb;50(2):273-83 - PubMed
  69. Bioinformatics. 2009 Aug 15;25(16):2078-9 - PubMed
  70. Nat Rev Immunol. 2010 Mar;10(3):182-92 - PubMed
  71. Gastroenterology. 2020 Mar;158(4):930-946.e1 - PubMed
  72. Genome Announc. 2014 Apr 10;2(2): - PubMed
  73. Nature. 2013 Dec 19;504(7480):446-50 - PubMed
  74. Cell Host Microbe. 2017 Dec 13;22(6):733-745.e5 - PubMed
  75. Cell Host Microbe. 2014 Mar 12;15(3):382-392 - PubMed

Publication Types

Grant support