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Wu J, Ning Y, Tan L, et al. Characteristics of the vaginal microbiome in women with premature ovarian insufficiency. J Ovarian Res. 2021;14(1):172doi: 10.1186/s13048-021-00923-9.
Wu, J., Ning, Y., Tan, L., Chen, Y., Huang, X., & Zhuo, Y. (2021). Characteristics of the vaginal microbiome in women with premature ovarian insufficiency. Journal of ovarian research, 14(1), 172. https://doi.org/10.1186/s13048-021-00923-9
Wu, Jiaman, et al. "Characteristics of the vaginal microbiome in women with premature ovarian insufficiency." Journal of ovarian research vol. 14,1 (2021): 172. doi: https://doi.org/10.1186/s13048-021-00923-9
Wu J, Ning Y, Tan L, Chen Y, Huang X, Zhuo Y. Characteristics of the vaginal microbiome in women with premature ovarian insufficiency. J Ovarian Res. 2021 Dec 08;14(1):172. doi: 10.1186/s13048-021-00923-9. PMID: 34879874; PMCID: PMC8655991.
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J Ovarian Res. 2021 Dec 08;14(1):172. doi: 10.1186/s13048-021-00923-9.

Characteristics of the vaginal microbiome in women with premature ovarian insufficiency.

Journal of ovarian research

Jiaman Wu, Yan Ning, Liya Tan, Yan Chen, Xingxian Huang, Yuanyuan Zhuo

Affiliations

  1. Department of Chinese Medicine, Affiliated Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, 518028, China.
  2. Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
  3. Department of Acupuncture and Moxibustion, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, 518033, China.
  4. Department of Acupuncture and Moxibustion, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, 518033, China. [email protected].

PMID: 34879874 PMCID: PMC8655991 DOI: 10.1186/s13048-021-00923-9

Abstract

PURPOSE: To investigate the relationship between vaginal microbial community structure and premature ovarian insufficiency (POI).

METHODS: Twenty-eight women with POI and 12 healthy women were recruited at Shenzhen Maternity and Child Healthcare Hospital between August and September 2020. Blood samples were collected for glucose tests and detection of sex hormone levels and vaginal secretions were collected for microbial group determination. Vaginal microbial community profiles were analysed by 16S rRNA gene sequencing using the Illumina MiSeq system (Illumina Inc., San Diego, CA, USA).

RESULTS: Compared to the controls, the serum levels of follicle-stimulating hormone, luteinizing hormone, testosterone, and the follicle-stimulating hormone/luteinizing hormone ratio, significantly increased, and oestradiol and anti-Müllerian hormone levels significantly decreased in women with POI. Higher weighted UniFrac values were observed in women with POI than in healthy women. Bacteria in the genera Lactobacillus, Brevundimonas, and Odoribacter were more abundant in the microbiomes of healthy women, while the quantity of bacteria in the genus Streptococcus was significantly increased in the microbiomes of women with POI. Moreover, these differences in microbes in women with POI were closely related to follicle-stimulating hormone, luteinizing hormone, oestradiol, and anti-Müllerian hormone levels and to the follicle-stimulating hormone/luteinizing hormone ratio.

CONCLUSIONS: Women with POI had altered vaginal microbial profiles compared to healthy controls. The alterations in their microbiomes were associated with serum hormone levels. These results will improve our understanding of the vaginal microbial community structure in women with POI.

TRIAL REGISTRATION: CHICTR, ChiCTR2000029576 . Registered 3 August 2020 - Retrospectively registered, https://www.chictr.org.cn/showproj.aspx?proj=48844 .

© 2021. The Author(s).

Keywords: 16S rRNA sequencing; Gonadal steroid hormones; Premature ovarian insufficiency; Vaginal microbiota

References

  1. Menopause. 2018 Nov;25(11):1321-1330 - PubMed
  2. Reprod Sci. 2012 Mar;19(3):235-42 - PubMed
  3. Am J Reprod Immunol. 2020 Apr;83(4):e13220 - PubMed
  4. Hum Reprod. 2002 Mar;17(3):659-65 - PubMed
  5. Eur J Clin Microbiol Infect Dis. 2021 Mar;40(3):651-656 - PubMed
  6. Nat Rev Immunol. 2015 Apr;15(4):217-30 - PubMed
  7. Microb Pathog. 2019 Nov;136:103692 - PubMed
  8. Best Pract Res Clin Obstet Gynaecol. 2019 Oct;60:42-55 - PubMed
  9. Gynecol Endocrinol. 2015 Jan;31(1):65-9 - PubMed
  10. Curr Opin Clin Nutr Metab Care. 2009 Nov;12(6):583-7 - PubMed
  11. Hum Reprod. 1999 Jul;14(7):1690-7 - PubMed
  12. Arch Endocrinol Metab. 2016 Nov-Dec;60(6):587-595 - PubMed
  13. Menopause. 2014 Oct;21(10):1063-8 - PubMed
  14. J Clin Invest. 2016 Jun 1;126(6):2049-63 - PubMed
  15. Best Pract Res Clin Endocrinol Metab. 2015 Jan;29(1):105-14 - PubMed
  16. Int Immunopharmacol. 2010 Jun;10(6):694-706 - PubMed
  17. J Midlife Health. 2015 Oct-Dec;6(4):147-53 - PubMed
  18. J Surg Res. 2003 Dec;115(2):325-44 - PubMed
  19. Front Immunol. 2019 Mar 01;10:358 - PubMed
  20. Am J Transl Res. 2013 Dec 01;6(1):91-101 - PubMed
  21. Nat Med. 2019 Jun;25(6):1012-1021 - PubMed
  22. Fertil Steril. 2020 Apr;113(4):845-852 - PubMed
  23. J Endocrinol Invest. 2016 Sep;39(9):983-90 - PubMed
  24. Methods Mol Biol. 2004;268:387-399 - PubMed
  25. Nat Biotechnol. 2020 Jun;38(6):685-688 - PubMed
  26. Nat Med. 2019 Jun;25(6):1001-1011 - PubMed
  27. Environ Sci Pollut Res Int. 2018 Feb;25(4):3423-3434 - PubMed
  28. Cell Metab. 2018 Oct 2;28(4):557-572.e6 - PubMed
  29. Microb Pathog. 2019 Nov;136:103696 - PubMed
  30. AIDS Res Ther. 2017 Sep 12;14(1):39 - PubMed
  31. J Reprod Immunol. 2014 Dec;106:67-75 - PubMed
  32. Am J Epidemiol. 2005 Dec 1;162(11):1089-97 - PubMed
  33. Am J Obstet Gynecol. 2021 Mar;224(3):251-257 - PubMed
  34. Cell Host Microbe. 2021 Jun 9;29(6):959-974.e7 - PubMed
  35. Genome Biol. 2011 Jun 24;12(6):R60 - PubMed
  36. Reprod Toxicol. 2000 Sep-Oct;14(5):377-84 - PubMed
  37. Reproduction. 2010 Nov;140(5):721-32 - PubMed
  38. J Immunol Res. 2019 Jan 2;2019:8069898 - PubMed
  39. BMC Microbiol. 2020 Jul 29;20(1):230 - PubMed
  40. J Endocrinol Invest. 2015 Jun;38(6):597-603 - PubMed
  41. Menopause. 2015 Jul;22(7):702-9 - PubMed

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