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Rui S, Yuan Y, Du C, et al. Comparison and Investigation of Exosomes Derived from Platelet-Rich Plasma Activated by Different Agonists. Cell Transplant. 2021;30:9636897211017833doi: 10.1177/09636897211017833.
Rui, S., Yuan, Y., Du, C., Song, P., Chen, Y., Wang, H., Fan, Y., Armstrong, D. G., Deng, W., & Li, L. (2021). Comparison and Investigation of Exosomes Derived from Platelet-Rich Plasma Activated by Different Agonists. Cell transplantation, 309636897211017833. https://doi.org/10.1177/09636897211017833
Rui, Shunli, et al. "Comparison and Investigation of Exosomes Derived from Platelet-Rich Plasma Activated by Different Agonists." Cell transplantation vol. 30 (2021): 9636897211017833. doi: https://doi.org/10.1177/09636897211017833
Rui S, Yuan Y, Du C, Song P, Chen Y, Wang H, Fan Y, Armstrong DG, Deng W, Li L. Comparison and Investigation of Exosomes Derived from Platelet-Rich Plasma Activated by Different Agonists. Cell Transplant. 2021 Jan-Dec;30:9636897211017833. doi: 10.1177/09636897211017833. PMID: 34006140; PMCID: PMC8138303.
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Cell Transplant. 2021 Jan-Dec;30:9636897211017833. doi: 10.1177/09636897211017833.

Comparison and Investigation of Exosomes Derived from Platelet-Rich Plasma Activated by Different Agonists.

Cell transplantation

Shunli Rui, Yi Yuan, Chenzhen Du, Peiyang Song, Yan Chen, Hongyan Wang, Yahan Fan, David G Armstrong, Wuquan Deng, Ling Li

Affiliations

  1. The Key Laboratory of Laboratory Medical Diagnostics in the Ministry of Education and Department of Clinical Biochemistry, College of Laboratory Medicine, 12550Chongqing Medical University, Chongqing, China.
  2. Department of Endocrinology, Multidisciplinary Diabetic Foot Medical Center, 159412Chongqing Emergency Medical Center, Chongqing University Central Hospital, Chongqing University, Chongqing, China.
  3. Department of Blood Transfusion, Southwest Hospital, Chongqing, China.
  4. Department of Surgery, Keck School of Medicine of the 12223University of Southern California, CA, USA.

PMID: 34006140 PMCID: PMC8138303 DOI: 10.1177/09636897211017833

Abstract

PRP-Exos are nanoscale cup-shaped vesicles that carry a variety of proteins, mRNAs, microRNAs, and other bioactive substances. PRP-Exos can be formed through several induction pathways, which determine their molecular profiles and facilitate their tailormade participation in intercellular communication. Currently, little is known on how the PRP-Exos activation method influences the quality and quantity of PRP-Exos. The present study aims to observe and analyze the number, profile, and growth factors of PRP-Exos through TEM, Nanoflow, and WB after PRP activation and compare the difference in function of PRP-Exos on HUVECs, with different stimuli (calcium gluconate, thrombin, or both). We found that PRP activated with both thrombin and calcium gluconate harvested the highest concentration of exosomes [(7.16 ± 0.46) × 10

Keywords: HUVECS; calcium gluconate; exosomes; platelet-rich plasma (PRP); thrombin

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