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Xu Z, Lin S, Tong Z, et al. Crocetin ameliorates non-alcoholic fatty liver disease by modulating mitochondrial dysfunction in L02 cells and zebrafish model. J Ethnopharmacol. 2021;285:114873doi: 10.1016/j.jep.2021.114873.
Xu, Z., Lin, S., Tong, Z., Chen, S., Cao, Y., Li, Q., Jiang, Y., Cai, W., Tong, Y., Zahra, B. S., & Wang, P. (2021). Crocetin ameliorates non-alcoholic fatty liver disease by modulating mitochondrial dysfunction in L02 cells and zebrafish model. Journal of ethnopharmacology, 285114873. https://doi.org/10.1016/j.jep.2021.114873
Xu, Zijin, et al. "Crocetin ameliorates non-alcoholic fatty liver disease by modulating mitochondrial dysfunction in L02 cells and zebrafish model." Journal of ethnopharmacology vol. 285 (2021): 114873. doi: https://doi.org/10.1016/j.jep.2021.114873
Xu Z, Lin S, Tong Z, Chen S, Cao Y, Li Q, Jiang Y, Cai W, Tong Y, Zahra BS, Wang P. Crocetin ameliorates non-alcoholic fatty liver disease by modulating mitochondrial dysfunction in L02 cells and zebrafish model. J Ethnopharmacol. 2021 Nov 27;285:114873. doi: 10.1016/j.jep.2021.114873. Epub 2021 Nov 27. PMID: 34848360.
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J Ethnopharmacol. 2021 Nov 27;285:114873. doi: 10.1016/j.jep.2021.114873. Epub 2021 Nov 27.

Crocetin ameliorates non-alcoholic fatty liver disease by modulating mitochondrial dysfunction in L02 cells and zebrafish model.

Journal of ethnopharmacology

Zijin Xu, Susu Lin, Zheren Tong, Suhong Chen, Yifeng Cao, Qiaoqiao Li, Yuli Jiang, Weijie Cai, Yingpeng Tong, Bathaie S Zahra, Ping Wang

Affiliations

  1. College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.
  2. Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, People's Republic of China.
  3. School of Life Sciences, Taizhou University, Taizhou, 318000, People's Republic of China.
  4. Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box. 14115-133, Tehran, Islamic Republic of Iran.
  5. College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China. Electronic address: [email protected].

PMID: 34848360 DOI: 10.1016/j.jep.2021.114873

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE: Traditional Chinese medicine considers that the etiology and pathogenesis of non-alcoholic fatty liver disease (NAFLD) are related to liver depression and qi stagnation. Saffron and its active ingredient, crocetin (CCT), are used for the treatment of metabolic diseases owing to their "Liver deobstruent" and "Liver tonic" effects. However, the effect of CCT on NAFLD has not been fully elucidated. In the present study, the effect and potential molecular mechanism of CCT were explored in both in vivo and in vitro models of NAFLD.

MATERIALS AND METHODS: CCT was isolated from saffron and purity and structure characterization were performed using HPLC, MS,

RESULTS: The levels of triglyceride (TG), total cholesterol (TC), malondialdehyde (MDA), and alanine/aspartate aminotransferases (ALT/AST) activities in FFA treated L02 cells were significantly reduced after CCT treatment. CCT treatment significantly increased ATP concentration, ΔΨm, and activities of superoxide dismutase (SOD), catalase (CAT), and cytochrome c oxidase (COX IV) in FFA treated L02 cells. TEM images showed restoration of mitochondrial morphology. CCT decreased ATP concentration and upregulated expression of B-cell lymphoma-2 (Bcl-2) and COX IV, whereas, CCT downregulated expression of BCL2-Associated X (Bax) and cleaved caspase-3 in TAA treated zebrafish. These findings indicated that mitochondrial dysfunction was alleviated after CCT treatment. Oil Red O staining of L02 cells and zebrafish showed that CCT treatment reversed the accumulation of lipid droplets.

CONCLUSION: In summary, CCT treatment effectively alleviated the symptoms of NAFLD and restored mitochondrial function in L02 cells and zebrafish NAFLD model.

Copyright © 2021 Elsevier B.V. All rights reserved.

Keywords: Crocetin; L02 cells; Mitochondrial function; NAFLD; Saffron; Zebrafish

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