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Hayashi KI, Arai K, Aoi Y, et al. The main oxidative inactivation pathway of the plant hormone auxin. Nat Commun. 2021;12(1):6752doi: 10.1038/s41467-021-27020-1.
Hayashi, K. I., Arai, K., Aoi, Y., Tanaka, Y., Hira, H., Guo, R., Hu, Y., Ge, C., Zhao, Y., Kasahara, H., & Fukui, K. (2021). The main oxidative inactivation pathway of the plant hormone auxin. Nature communications, 12(1), 6752. https://doi.org/10.1038/s41467-021-27020-1
Hayashi, Ken-Ichiro, et al. "The main oxidative inactivation pathway of the plant hormone auxin." Nature communications vol. 12,1 (2021): 6752. doi: https://doi.org/10.1038/s41467-021-27020-1
Hayashi KI, Arai K, Aoi Y, Tanaka Y, Hira H, Guo R, Hu Y, Ge C, Zhao Y, Kasahara H, Fukui K. The main oxidative inactivation pathway of the plant hormone auxin. Nat Commun. 2021 Nov 22;12(1):6752. doi: 10.1038/s41467-021-27020-1. PMID: 34811366; PMCID: PMC8608799.
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Nat Commun. 2021 Nov 22;12(1):6752. doi: 10.1038/s41467-021-27020-1.

The main oxidative inactivation pathway of the plant hormone auxin.

Nature communications

Ken-Ichiro Hayashi, Kazushi Arai, Yuki Aoi, Yuka Tanaka, Hayao Hira, Ruipan Guo, Yun Hu, Chennan Ge, Yunde Zhao, Hiroyuki Kasahara, Kosuke Fukui

Affiliations

  1. Department of Biochemistry, Okayama University of Science, Okayama, 700-0005, Japan. [email protected].
  2. Department of Biochemistry, Okayama University of Science, Okayama, 700-0005, Japan.
  3. Department of Biological Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
  4. Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
  5. Section of Cell and Developmental Biology, University of California San Diego, Gilman Dr. La Jolla, San Diego, CA, 92093-0116, USA.
  6. RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, 230-0045, Japan.

PMID: 34811366 PMCID: PMC8608799 DOI: 10.1038/s41467-021-27020-1

Abstract

Inactivation of the phytohormone auxin plays important roles in plant development, and several enzymes have been implicated in auxin inactivation. In this study, we show that the predominant natural auxin, indole-3-acetic acid (IAA), is mainly inactivated via the GH3-ILR1-DAO pathway. IAA is first converted to IAA-amino acid conjugates by GH3 IAA-amidosynthetases. The IAA-amino acid conjugates IAA-aspartate (IAA-Asp) and IAA-glutamate (IAA-Glu) are storage forms of IAA and can be converted back to IAA by ILR1/ILL amidohydrolases. We further show that DAO1 dioxygenase irreversibly oxidizes IAA-Asp and IAA-Glu into 2-oxindole-3-acetic acid-aspartate (oxIAA-Asp) and oxIAA-Glu, which are subsequently hydrolyzed by ILR1 to release inactive oxIAA. This work established a complete pathway for the oxidative inactivation of auxin and defines the roles played by auxin homeostasis in plant development.

© 2021. The Author(s).

References

  1. Biochem Biophys Res Commun. 2020 Jul 5;527(4):1033-1038 - PubMed
  2. Plant J. 2002 Jan;29(2):153-68 - PubMed
  3. Proc Natl Acad Sci U S A. 2018 Jun 26;115(26):6864-6869 - PubMed
  4. Plant Physiol. 2004 Jun;135(2):978-88 - PubMed
  5. J Integr Plant Biol. 2014 Apr;56(4):343-9 - PubMed
  6. Proc Natl Acad Sci U S A. 2016 Sep 27;113(39):11010-5 - PubMed
  7. Plant Physiol. 2016 Jul;171(3):1794-800 - PubMed
  8. Biochem Biophys Res Commun. 2020 Nov 5;532(2):244-250 - PubMed
  9. Science. 2002 Apr 5;296(5565):141-5 - PubMed
  10. Plant Cell. 2005 Feb;17(2):616-27 - PubMed
  11. Science. 1995 Jun 23;268(5218):1745-8 - PubMed
  12. Nat Plants. 2016 Mar 21;2:16025 - PubMed
  13. J Exp Bot. 2011 Mar;62(6):1757-73 - PubMed
  14. Plant Cell. 1997 Nov;9(11):1963-71 - PubMed
  15. J Biol Chem. 2002 Jun 7;277(23):20446-52 - PubMed
  16. J Exp Bot. 2013 Jun;64(9):2541-55 - PubMed
  17. Int J Mol Sci. 2019 Sep 09;20(18): - PubMed
  18. Nat Commun. 2020 May 1;11(1):2143 - PubMed
  19. Plant Physiol. 1988;86:868-72 - PubMed
  20. J Struct Biol. 2020 Dec 1;212(3):107632 - PubMed
  21. Proc Natl Acad Sci U S A. 2011 Nov 8;108(45):18512-7 - PubMed
  22. Biosci Biotechnol Biochem. 2005 Apr;69(4):778-83 - PubMed
  23. New Phytol. 2020 Jun;226(6):1753-1765 - PubMed
  24. Plant Cell. 1990 Nov;2(11):1071-80 - PubMed
  25. Plant Cell. 2005 Oct;17(10):2693-704 - PubMed
  26. J Exp Bot. 2017 Jun 1;68(12):3145-3154 - PubMed
  27. Plant Physiol. 2001 Dec;127(4):1845-53 - PubMed
  28. Plant Cell. 1999 Mar;11(3):365-76 - PubMed
  29. Plant J. 2002 Nov;32(4):573-83 - PubMed
  30. Plant Cell Physiol. 2014 Jan;55(1):218-28 - PubMed
  31. Dev Cell. 2005 Jul;9(1):109-19 - PubMed
  32. Plant Physiol. 2021 Sep 4;187(1):103-115 - PubMed
  33. Plant Cell. 2013 Oct;25(10):3858-70 - PubMed
  34. Proc Natl Acad Sci U S A. 2016 Sep 27;113(39):11016-21 - PubMed
  35. Dev Cell. 2013 Oct 14;27(1):113-22 - PubMed
  36. Proc Natl Acad Sci U S A. 2016 Sep 27;113(39):11022-7 - PubMed
  37. Annu Rev Plant Biol. 2018 Apr 29;69:417-435 - PubMed
  38. Plant Cell Physiol. 2015 Aug;56(8):1641-54 - PubMed
  39. Proc Natl Acad Sci U S A. 2011 Nov 8;108(45):18518-23 - PubMed
  40. Planta. 2007 Jun;226(1):21-34 - PubMed

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