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Phytochemistry. 2022 Feb;194:113022. doi: 10.1016/j.phytochem.2021.113022. Epub 2021 Nov 23.

Structure and activity of a novel robust peroxidase from Alkanna frigida cell culture.

Phytochemistry

Saeed Soleimani Asl, Ali Asghar Karkhane, Javad Zamani Amirzakaria, Kambiz Akbari Noghabi, Jaleh Ghashghaie, Kamahldin Haghbeen

Affiliations

  1. National Institute for Genetic Engineering and Biotechnology, Tehran, Iran.
  2. Laboratoire d'Ecologie, Systématique et Evolution (ESE), Université de Paris-Sud, CNRS, AgroParisTech, Université de Paris-Saclay, 91400 Orsay, France.
  3. National Institute for Genetic Engineering and Biotechnology, Tehran, Iran. Electronic address: [email protected].

PMID: 34826793 DOI: 10.1016/j.phytochem.2021.113022

Abstract

Demands for peroxidases (POX)s with diverse physicochemical properties have steadily grown as more applications of POXs are demonstrated. Plants are among the best sources of versatile POXs, and plant biotechnology, as an agricultural hassle-free technology, promises to circumvent the limitations of natural resource exploitation and to address the demands. Following this trend, it was shown that POX production steadily increased during the 31-day subculture of Alkanna frigida (from Boraginaceae) callus on Murashige-Skoog medium containing 2,4-dichlorophenoxyacetic acid (10

Copyright © 2021 Elsevier Ltd. All rights reserved.

Keywords: Active site pocket; Alkanna frigida; Boraginaceae; Callus; Functional stability; Peanut peroxidase; Suicide substrate

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