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Bartolomé J, Romeo SC, Dorado-Silva M, et al. DNase activity in human seminal plasma and follicular fluid and its inhibition by follicular fluid chelating agents. Reprod Biomed Online. 2021;43(6):1079-1086doi: 10.1016/j.rbmo.2021.09.015.
Bartolomé, J., Romeo, S. C., Dorado-Silva, M., García de la Vega, C., López, C., Sánchez-Martín, P., Johnston, S., & Gosálvez, J. (2021). DNase activity in human seminal plasma and follicular fluid and its inhibition by follicular fluid chelating agents. Reproductive biomedicine online, 43(6), 1079-1086. https://doi.org/10.1016/j.rbmo.2021.09.015
Bartolomé, Javier, et al. "DNase activity in human seminal plasma and follicular fluid and its inhibition by follicular fluid chelating agents." Reproductive biomedicine online vol. 43,6 (2021): 1079-1086. doi: https://doi.org/10.1016/j.rbmo.2021.09.015
Bartolomé J, Romeo SC, Dorado-Silva M, García de la Vega C, López C, Sánchez-Martín P, Johnston S, Gosálvez J. DNase activity in human seminal plasma and follicular fluid and its inhibition by follicular fluid chelating agents. Reprod Biomed Online. 2021 Dec;43(6):1079-1086. doi: 10.1016/j.rbmo.2021.09.015. Epub 2021 Sep 26. PMID: 34753679.
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Reprod Biomed Online. 2021 Dec;43(6):1079-1086. doi: 10.1016/j.rbmo.2021.09.015. Epub 2021 Sep 26.

DNase activity in human seminal plasma and follicular fluid and its inhibition by follicular fluid chelating agents.

Reproductive biomedicine online

Javier Bartolomé, Sandra Claver Romeo, Mónica Dorado-Silva, Carlos García de la Vega, Carmen López, Pascual Sánchez-Martín, Stephen Johnston, Jaime Gosálvez

Affiliations

  1. Unit of Genetics, Department of Biology, Universidad Autónoma de Madrid, Cantoblanco, Madrid 28049, Spain; Halotech DNA, Cantoblanco, Madrid 28049, Spain.
  2. Halotech DNA, Cantoblanco, Madrid 28049, Spain.
  3. Ginemed, Clinic for Assisted Reproduction Sevilla 41010, Spain.
  4. Unit of Genetics, Department of Biology, Universidad Autónoma de Madrid, Cantoblanco, Madrid 28049, Spain.
  5. School of Agriculture and Food Sciences, University of Queensland, Gatton 4343, Australia. Electronic address: [email protected].

PMID: 34753679 DOI: 10.1016/j.rbmo.2021.09.015

Abstract

RESEARCH QUESTION: What is the mechanism by which human follicular fluid inhibits seminal plasma DNase activity?

DESIGN: Human genomic DNA was incubated with human follicular fluid and seminal plasma (reaction mixture) under different experimental conditions; increasing volumes of human follicular fluid; proteinase K digested or heat inactivated human follicular fluid; and the addition of Ca

RESULTS: Increasing volume of human follicular fluid resulted in a dose-dependent inhibition of seminal plasma DNase activity. Inhibition was not caused by proteins in the human follicular fluid as digestion with proteinase K or heat inactivation of human follicular fluid failed to abolish its inhibitory effect. Addition of divalent cations resulted in a reversion of the inhibitory effect, providing evidence that human follicular fluid inhibition of seminal plasma DNase activity seems to be mediated by a compound with chelating activity. Furthermore, incubation of genomic DNA with human follicular fluid in the presence of divalent cations served to elicit the existence of DNase activity.

CONCLUSIONS: Human follicular fluid seems to contain a molecule or molecules with chelating capacity that inhibits DNase activity of both follicular fluid and seminal plasma. Our findings provide new insight to understanding sperm preservation and the physiology of fertilization biology.

Copyright © 2021 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

Keywords: DNase activity; Human follicular fluid; Seminal plasma; Sperm DNA fragmentation

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