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Rabe AJ, Tan YY, Larue RC, et al. Prototype Foamy Virus Integrase Displays Unique Biochemical Activities among Retroviral Integrases. Biomolecules. 2021;11(12)doi: 10.3390/biom11121910.
Rabe, A. J., Tan, Y. Y., Larue, R. C., & Yoder, K. E. (2021). Prototype Foamy Virus Integrase Displays Unique Biochemical Activities among Retroviral Integrases. Biomolecules, 11(12), . https://doi.org/10.3390/biom11121910
Rabe, Anthony J, et al. "Prototype Foamy Virus Integrase Displays Unique Biochemical Activities among Retroviral Integrases." Biomolecules vol. 11,12 (2021). doi: https://doi.org/10.3390/biom11121910
Rabe AJ, Tan YY, Larue RC, Yoder KE. Prototype Foamy Virus Integrase Displays Unique Biochemical Activities among Retroviral Integrases. Biomolecules. 2021 Dec 20;11(12). doi: 10.3390/biom11121910. PMID: 34944553; PMCID: PMC8699820.
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Biomolecules. 2021 Dec 20;11(12). doi: 10.3390/biom11121910.

Prototype Foamy Virus Integrase Displays Unique Biochemical Activities among Retroviral Integrases.

Biomolecules

Anthony J Rabe, Yow Yong Tan, Ross C Larue, Kristine E Yoder

Affiliations

  1. Department of Cancer Biology and Genetics, The Ohio State University College of Medicine, Columbus, OH 43026, USA.
  2. Center for Retrovirus Research, The Ohio State University, Columbus, OH 43026, USA.
  3. Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43026, USA.

PMID: 34944553 PMCID: PMC8699820 DOI: 10.3390/biom11121910

Abstract

Integrases of different retroviruses assemble as functional complexes with varying multimers of the protein. Retroviral integrases require a divalent metal cation to perform one-step transesterification catalysis. Tetrameric prototype foamy virus (PFV) intasomes assembled from purified integrase and viral DNA oligonucleotides were characterized for their activity in the presence of different cations. While most retroviral integrases are inactive in calcium, PFV intasomes appear to be uniquely capable of catalysis in calcium. The PFV intasomes also contrast with other retroviral integrases by displaying an inverse correlation of activity with increasing manganese beginning at relatively low concentrations. The intasomes were found to be significantly more active in the presence of chloride co-ions compared to acetate. While HIV-1 integrase appears to commit to a target DNA within 20 s, PFV intasomes do not commit to target DNA during their reaction lifetime. Together, these data highlight the unique biochemical activities of PFV integrase compared to other retroviral integrases.

Keywords: divalent cation; integrase; prototype foamy virus; retrovirus

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