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Sakib S, Voigt A, de Lima E Martins Lara N, et al. The Proliferation of Pre-Pubertal Porcine Spermatogonia in Stirred Suspension Bioreactors Is Partially Mediated by the Wnt/β-Catenin Pathway. Int J Mol Sci. 2021;22(24)doi: 10.3390/ijms222413549.
Sakib, S., Voigt, A., de Lima E Martins Lara, N., Su, L., Ungrin, M., Rancourt, D., & Dobrinski, I. (2021). The Proliferation of Pre-Pubertal Porcine Spermatogonia in Stirred Suspension Bioreactors Is Partially Mediated by the Wnt/β-Catenin Pathway. International journal of molecular sciences, 22(24), . https://doi.org/10.3390/ijms222413549
Sakib, Sadman, et al. "The Proliferation of Pre-Pubertal Porcine Spermatogonia in Stirred Suspension Bioreactors Is Partially Mediated by the Wnt/β-Catenin Pathway." International journal of molecular sciences vol. 22,24 (2021). doi: https://doi.org/10.3390/ijms222413549
Sakib S, Voigt A, de Lima E Martins Lara N, Su L, Ungrin M, Rancourt D, Dobrinski I. The Proliferation of Pre-Pubertal Porcine Spermatogonia in Stirred Suspension Bioreactors Is Partially Mediated by the Wnt/β-Catenin Pathway. Int J Mol Sci. 2021 Dec 17;22(24). doi: 10.3390/ijms222413549. PMID: 34948348; PMCID: PMC8708394.
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Int J Mol Sci. 2021 Dec 17;22(24). doi: 10.3390/ijms222413549.

The Proliferation of Pre-Pubertal Porcine Spermatogonia in Stirred Suspension Bioreactors Is Partially Mediated by the Wnt/β-Catenin Pathway.

International journal of molecular sciences

Sadman Sakib, Anna Voigt, Nathalia de Lima E Martins Lara, Lin Su, Mark Ungrin, Derrick Rancourt, Ina Dobrinski

Affiliations

  1. Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada.
  2. Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 1N4, Canada.
  3. Biomedical Engineering Graduate Program, University of Calgary, Calgary, AB T2N 1N4, Canada.
  4. Department of Oncology and Medical Genetics, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N2, Canada.

PMID: 34948348 PMCID: PMC8708394 DOI: 10.3390/ijms222413549

Abstract

Male survivors of childhood cancer are at risk of suffering from infertility in adulthood because of gonadotoxic chemotherapies. For adult men, sperm collection and preservation are routine procedures prior to treatment; however, this is not an option for pre-pubertal children. From young boys, a small biopsy may be taken before chemotherapy, and spermatogonia may be propagated in vitro for future transplantation to restore fertility. A robust system that allows for scalable expansion of spermatogonia within a controlled environment is therefore required. Stirred suspension culture has been applied to different types of stem cells but has so far not been explored for spermatogonia. Here, we report that pre-pubertal porcine spermatogonia proliferate more in bioreactor suspension culture, compared with static culture. Interestingly, oxygen tension provides an avenue to modulate spermatogonia status, with culture under 10% oxygen retaining a more undifferentiated state and reducing proliferation in comparison with the conventional approach of culturing under ambient oxygen levels. Spermatogonia grown in bioreactors upregulate the Wnt/ β-catenin pathway, which, along with enhanced gas and nutrient exchange observed in bioreactor culture, may synergistically account for higher spermatogonia proliferation. Therefore, stirred suspension bioreactors provide novel platforms to culture spermatogonia in a scalable manner and with minimal handling.

Keywords: bioreactor; culture; germ cell; spermatogonia; stirred suspension bioreactor

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