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Lee JG, Lee J, Lee AR, et al. Impact of short-chain fatty acid supplementation on gut inflammation and microbiota composition in a murine colitis model. J Nutr Biochem. 2021;101:108926doi: 10.1016/j.jnutbio.2021.108926.
Lee, J. G., Lee, J., Lee, A. R., Jo, S. V., Park, C. H., Han, D. S., & Eun, C. S. (2021). Impact of short-chain fatty acid supplementation on gut inflammation and microbiota composition in a murine colitis model. The Journal of nutritional biochemistry, 101108926. https://doi.org/10.1016/j.jnutbio.2021.108926
Lee, Jae Gon, et al. "Impact of short-chain fatty acid supplementation on gut inflammation and microbiota composition in a murine colitis model." The Journal of nutritional biochemistry vol. 101 (2021): 108926. doi: https://doi.org/10.1016/j.jnutbio.2021.108926
Lee JG, Lee J, Lee AR, Jo SV, Park CH, Han DS, Eun CS. Impact of short-chain fatty acid supplementation on gut inflammation and microbiota composition in a murine colitis model. J Nutr Biochem. 2021 Nov 27;101:108926. doi: 10.1016/j.jnutbio.2021.108926. Epub 2021 Nov 27. PMID: 34848335.
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J Nutr Biochem. 2021 Nov 27;101:108926. doi: 10.1016/j.jnutbio.2021.108926. Epub 2021 Nov 27.

Impact of short-chain fatty acid supplementation on gut inflammation and microbiota composition in a murine colitis model.

The Journal of nutritional biochemistry

Jae Gon Lee, Jiyoung Lee, A-Reum Lee, Su Vin Jo, Chan Hyuk Park, Dong Soo Han, Chang Soo Eun

Affiliations

  1. Department of Internal Medicine, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, Korea.
  2. Department of Internal Medicine, Hanyang University Guri Hospital, Hanyang University College of Medicine, Guri, Korea.
  3. Department of Internal Medicine, Hanyang University Guri Hospital, Hanyang University College of Medicine, Guri, Korea. Electronic address: [email protected].

PMID: 34848335 DOI: 10.1016/j.jnutbio.2021.108926

Abstract

Short-chain fatty acids (SCFAs) play a pivotal role in maintaining intestinal homeostasis. We aimed to investigate the effects of SCFA supplementation on gut inflammation and microbiota composition in a murine colitis model. Mice were fed with sodium butyrate or a mixture of SCFAs in the drinking water for 2 weeks, followed by 2% dextran sulfate sodium (DSS) for 7 d. After euthanasia, mouse colons were extracted to examine histological findings. Flow cytometry of the mouse colon tissues was performed to assess T cell differentiation. Changes in gut microbiota were assessed by high-throughput sequencing of the mouse feces. There were no significant differences in weight change, colonic length, or histologic inflammation score between the DSS, butyrate, and SCFA mix groups. However, flow cytometry revealed that both the expression of CD4+Foxp3+ regulatory T cells and of IL-17-producing T cells were increased in the butyrate and SCFA mix groups. Microbial compositions of the butyrate and SCFA mix groups were significantly different from those of the control and DSS groups in principal coordinate analysis. Relative abundances of the phyla Verrucomicrobia and Proteobacteria, species Akkermansia muciniphila and Escherichia fergusonii were increased in the butyrate and SCFA mix groups. Genera Roseburia and Lactobacillus showed a negative correlation with the degree of colitis, whereas genera Escherichia and Mucispirillum showed a positive correlation. SCFA supplementation did not result in a significant reduction in colon inflammation, but it promoted both regulatory T cell and IL-17-producing T cell expression, and increased both protective and aggressive gut microbiota.

Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Keywords: Butyrates; Colitis; Gut microbiota; Inflammatory bowel disease; Short-chain fatty acids

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