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Wang K, Wang S, Chen Y, et al. Single-cell transcriptomic analysis of somatosensory neurons uncovers temporal development of neuropathic pain. Cell Res. 2021;31(8):904-918doi: 10.1038/s41422-021-00479-9.
Wang, K., Wang, S., Chen, Y., Wu, D., Hu, X., Lu, Y., Wang, L., Bao, L., Li, C., & Zhang, X. (2021). Single-cell transcriptomic analysis of somatosensory neurons uncovers temporal development of neuropathic pain. Cell research, 31(8), 904-918. https://doi.org/10.1038/s41422-021-00479-9
Wang, Kaikai, et al. "Single-cell transcriptomic analysis of somatosensory neurons uncovers temporal development of neuropathic pain." Cell research vol. 31,8 (2021): 904-918. doi: https://doi.org/10.1038/s41422-021-00479-9
Wang K, Wang S, Chen Y, Wu D, Hu X, Lu Y, Wang L, Bao L, Li C, Zhang X. Single-cell transcriptomic analysis of somatosensory neurons uncovers temporal development of neuropathic pain. Cell Res. 2021 Aug;31(8):904-918. doi: 10.1038/s41422-021-00479-9. Epub 2021 Mar 10. PMID: 33692491; PMCID: PMC8324866.
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Cell Res. 2021 Aug;31(8):904-918. doi: 10.1038/s41422-021-00479-9. Epub 2021 Mar 10.

Single-cell transcriptomic analysis of somatosensory neurons uncovers temporal development of neuropathic pain.

Cell research

Kaikai Wang, Sashuang Wang, Yan Chen, Dan Wu, Xinyu Hu, Yingjin Lu, Liping Wang, Lan Bao, Changlin Li, Xu Zhang

Affiliations

  1. Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China.
  2. School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
  3. University of Chinese Academy of Sciences, Beijing, 100049, China.
  4. Research Unit of Pain, Chinese Academy of Medical Sciences, Institute of Brain-Intelligence Science and Technology, Zhangjiang Lab, Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, 200031, China.
  5. Shanghai Clinical Research Center, Chinese Academy of Sciences, Xuhui Central Hospital, Shanghai, 200031, China.
  6. Shenzhen Key Lab of Neuropsychiatric Modulation, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
  7. State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China.
  8. Research Unit of Pain, Chinese Academy of Medical Sciences, Institute of Brain-Intelligence Science and Technology, Zhangjiang Lab, Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, 200031, China. [email protected].
  9. Shanghai Clinical Research Center, Chinese Academy of Sciences, Xuhui Central Hospital, Shanghai, 200031, China. [email protected].
  10. Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China. [email protected].
  11. School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China. [email protected].
  12. University of Chinese Academy of Sciences, Beijing, 100049, China. [email protected].
  13. Research Unit of Pain, Chinese Academy of Medical Sciences, Institute of Brain-Intelligence Science and Technology, Zhangjiang Lab, Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, 200031, China. [email protected].

PMID: 33692491 PMCID: PMC8324866 DOI: 10.1038/s41422-021-00479-9

Abstract

Peripheral nerve injury could lead to chronic neuropathic pain. Understanding transcriptional changes induced by nerve injury could provide fundamental insights into the complex pathogenesis of neuropathic pain. Gene expression profiles of dorsal root ganglia (DRG) in neuropathic pain condition have been studied. However, little is known about transcriptomic changes in individual DRG neurons after peripheral nerve injury. Here we performed single-cell RNA sequencing on dissociated mouse DRG cells after spared nerve injury (SNI). In addition to DRG neuron types that are found under physiological conditions, we identified three SNI-induced neuronal clusters (SNIICs) characterized by the expression of Atf3/Gfra3/Gal (SNIIC1), Atf3/Mrgprd (SNIIC2) and Atf3/S100b/Gal (SNIIC3). These SNIICs originated from Cldn9

© 2021. The Author(s), under exclusive licence to Center for Excellence in Molecular Cell Science, CAS.

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