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He S, Kou K, O'Shea C, et al. A dataset of dual calcium and voltage optical mapping in healthy and hypertrophied murine hearts. Sci Data. 2021;8(1):314doi: 10.1038/s41597-021-01085-5.
He, S., Kou, K., O'Shea, C., Chen, T., Mu-U-Min, R., Dong, R., Ren, H., Zhou, X., Fan, Z., Tan, X., Pavlovic, D., Ou, X., & Lei, M. (2021). A dataset of dual calcium and voltage optical mapping in healthy and hypertrophied murine hearts. Scientific data, 8(1), 314. https://doi.org/10.1038/s41597-021-01085-5
He, Shicheng, et al. "A dataset of dual calcium and voltage optical mapping in healthy and hypertrophied murine hearts." Scientific data vol. 8,1 (2021): 314. doi: https://doi.org/10.1038/s41597-021-01085-5
He S, Kou K, O'Shea C, Chen T, Mu-U-Min R, Dong R, Ren H, Zhou X, Fan Z, Tan X, Pavlovic D, Ou X, Lei M. A dataset of dual calcium and voltage optical mapping in healthy and hypertrophied murine hearts. Sci Data. 2021 Dec 16;8(1):314. doi: 10.1038/s41597-021-01085-5. PMID: 34916511; PMCID: PMC8677726.
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Sci Data. 2021 Dec 16;8(1):314. doi: 10.1038/s41597-021-01085-5.

A dataset of dual calcium and voltage optical mapping in healthy and hypertrophied murine hearts.

Scientific data

Shicheng He, Kun Kou, Christopher O'Shea, Tangting Chen, Razik Mu-U-Min, Ruirui Dong, Huiying Ren, Xiaolin Zhou, Zhongcai Fan, Xiaoqiu Tan, Davor Pavlovic, Xianhong Ou, Ming Lei

Affiliations

  1. Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, China.
  2. Department of Cardiovascular Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, China.
  3. Institute of Cardiovascular Sciences, University of Birmingham, Birmingham, United Kingdom.
  4. Department of Pharmacology, University of Oxford, Oxford, United Kingdom.
  5. Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, China. [email protected].
  6. Department of Cardiovascular Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, China. [email protected].
  7. Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, China. [email protected].
  8. Department of Pharmacology, University of Oxford, Oxford, United Kingdom. [email protected].

PMID: 34916511 PMCID: PMC8677726 DOI: 10.1038/s41597-021-01085-5

Abstract

Pathological hypertrophy underlies sudden cardiac death due to its high incidence of occurrence of ventricular arrhythmias. The alteration of transmural electrophysiological properties in hypertrophic cardiac murine tissue has never been explored previously. In this dataset, we have for the first time conducted high-throughput simultaneous optical imaging of transmembrane potential and calcium transients (CaT) throughout the entire hypertrophic murine hearts at high temporal and spatial resolution. Using ElectroMap, we have conducted multiple parameters analysis including action potential duration/calcium transient duration, conduction velocity, alternans and diastolic interval. Voltage-calcium latency was measured as time difference between action potential and CaT peak. The dataset therefore provides the first high spatial resolution transmural electrophysiological profiling of the murine heart, allowing interrogation of mechanisms driving ventricular arrhythmias associated with pathological hypertrophy. The dataset allows for further reuse and detailed analyses of geometrical, topological and functional analyses and reconstruction of 2-dimensional and 3-dimentional models.

© 2021. Crown.

References

  1. Front Physiol. 2018 Jan 23;9:16 - PubMed
  2. Biophys J. 2012 Jan 4;102(1):30-8 - PubMed
  3. Prog Biophys Mol Biol. 2020 Nov;157:84-93 - PubMed
  4. Front Physiol. 2019 Oct 11;10:1295 - PubMed
  5. J Vis Exp. 2017 Sep 25;(127): - PubMed
  6. Curr Cardiol Rep. 2015 Jul;17(7):606 - PubMed
  7. Sci Rep. 2019 Feb 4;9(1):1389 - PubMed
  8. FASEB J. 2019 Nov;33(11):12240-12252 - PubMed
  9. J Vis Exp. 2019 Jun 4;(148): - PubMed
  10. Front Physiol. 2019 Jun 25;10:755 - PubMed
  11. Circulation. 2011 Dec 13;124(24):2702-15 - PubMed
  12. IEEE Trans Biomed Eng. 1998 May;45(5):563-71 - PubMed
  13. J Physiol. 2018 Sep;596(17):3951-3965 - PubMed

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