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Hanyu Y, Kato M. High-yield expression of periplasmic single-chain variable fragments by solid . Biotechniques. 2021;72(1):29-32doi: 10.2144/btn-2021-0093.
Hanyu, Y., & Kato, M. (2022). High-yield expression of periplasmic single-chain variable fragments by solid . BioTechniques, 72(1), 29-32. https://doi.org/10.2144/btn-2021-0093
Hanyu, Yoshiro, and Kato, Mieko. "High-yield expression of periplasmic single-chain variable fragments by solid ." BioTechniques vol. 72,1 (2022): 29-32. doi: https://doi.org/10.2144/btn-2021-0093
Hanyu Y, Kato M. High-yield expression of periplasmic single-chain variable fragments by solid . Biotechniques. 2022 Jan;72(1):29-32. doi: 10.2144/btn-2021-0093. Epub 2021 Nov 29. PMID: 34841891.
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Biotechniques. 2022 Jan;72(1):29-32. doi: 10.2144/btn-2021-0093. Epub 2021 Nov 29.

High-yield expression of periplasmic single-chain variable fragments by solid .

BioTechniques

Yoshiro Hanyu, Mieko Kato

Affiliations

  1. Biomaterials Research Group, Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, 305-8566, Japan.
  2. Department of Biochemistry, Bio-Peak Co., Ltd., 584-70 Shimonojo, Takasaki, 370-0854, Japan.

PMID: 34841891 DOI: 10.2144/btn-2021-0093

Abstract

High-yield expression of quality antibody fragments is indispensable for research and diagnosis. Most recombinant antibody fragments are expressed in

Keywords: E. coli; antibody; expression; periplasm; scFv; solid culture

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