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Showing 1 to 12 of 203 entries
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Short-read, high-throughput sequencing technology for STR genotyping.

BioTechniques. Rapid dispatches

Bornman DM, Hester ME, Schuetter JM, Kasoji MD, Minard-Smith A, Barden CA, Nelson SC, Godbold GD, Baker CH, Yang B, Walther JE, Tornes IE, Yan PS, Rodriguez B, Bundschuh R, Dickens ML, Young BA, Faith SA.
PMID: 25621315
Biotech Rapid Dispatches. 2012 Apr;2012:1-6.

DNA-based methods for human identification principally rely upon genotyping of short tandem repeat (STR) loci. Electrophoretic-based techniques for variable-length classification of STRs are universally utilized, but are limited in that they have relatively low throughput and do not yield...

The Fab Fragment of a Human Anti-Siglec-9 Monoclonal Antibody Suppresses LPS-Induced Inflammatory Responses in Human Macrophages.

Frontiers in immunology

Chu S, Zhu X, You N, Zhang W, Zheng F, Cai B, Zhou T, Wang Y, Sun Q, Yang Z, Zhang X, Wang C, Nie S, Zhu J, Wang M.
PMID: 28082984
Front Immunol. 2016 Dec 26;7:649. doi: 10.3389/fimmu.2016.00649. eCollection 2016.

Sepsis is a major cause of death for hospitalized patients and is characterized by massive overreaction of immune responses to invading pathogens which is mediated by cytokines. For decades, there has been no effective treatment for sepsis. Sialic acid-binding,...

Biotechniques for improving acid aluminum tolerance in alfalfa.

Plant cell reports

Kamp-Glass M, Powell D, Reddy GB, Baligar VC, Wright RJ.
PMID: 24201792
Plant Cell Rep. 1993 Aug;12(10):590-2. doi: 10.1007/BF00233067.

Alfalfa (Medicago sativa L.), cultivars ARC, Regen Y and Saranac were selected in vitro in a recently developed acid/aluminum toxic media. The new media produced higher initiation rates and higher fresh callus weights than those obtainable with the media...

Impossible problems.

BioTechniques

Webb WW.
PMID: 15884665
Biotechniques. 2005 Apr;38(4):515. doi: 10.2144/05384sp01.

No abstract available.

Returning to diagnostic basics.

BioTechniques

Webb SA.
PMID: 20629269
Biotechniques. 2010 Jul;49(1):491-3. doi: 10.2144/000113461.

No abstract available.

Antitumor activity of a newly developed monoclonal antibody against ROR1 in ovarian cancer cells.

Oncotarget

Yin Z, Gao M, Chu S, Su Y, Ye C, Wang Y, Pan Z, Wang Z, Zhang H, Tong H, Zhu J.
PMID: 29212222
Oncotarget. 2017 Oct 07;8(55):94210-94222. doi: 10.18632/oncotarget.21618. eCollection 2017 Nov 07.

Receptor-tyrosine-kinase-like Orphan Receptor 1 (ROR1) is a tyrosine-protein kinase transmembrane receptor and ROR1 overexpression is associated with a poor prognosis in various cancers, including ovarian cancer. Targeting of ROR1 has been evaluated as a novel cancer therapy strategy. This...

The killing effect of novel bi-specific Trop2/PD-L1 CAR-T cell targeted gastric cancer.

American journal of cancer research

Zhao W, Jia L, Zhang M, Huang X, Qian P, Tang Q, Zhu J, Feng Z.
PMID: 31497363
Am J Cancer Res. 2019 Aug 01;9(8):1846-1856. eCollection 2019.

Due to their high heterogeneity and complex tumor microenvironment, the treatment of solid tumors by CAR-T cell technology is limited. This study developed bi-specific Trop2/PD-L1 specific third-generation CAR-T cells by lentiviral infection. The specific killing ability of the bi-specific...

Opening up to open access.

BioTechniques

[No authors listed]
PMID: 30987441
Biotechniques. 2019 Apr;66(4):107. doi: 10.2144/btn-2019-0028.

No abstract available.

Simple, inexpensive and RNase-free purification of plasmid DNA by fractional precipitation with isopropanol.

BioTechniques

Paalme V, Speek M.
PMID: 34392702
Biotechniques. 2021 Sep;71(3):490-494. doi: 10.2144/btn-2021-0018. Epub 2021 Aug 16.

We present a modified alkaline lysis method for purification of plasmid DNA (pDNA) from bacterial extract using fractional precipitation with isopropanol (FPI). This method includes two successive precipitations with 0.33 and 0.36 volumes of isopropanol and separates pDNA from...

Comparative Analysis of Blood-Derived Endothelial Cells for Designing Next-Generation Personalized Organ-on-Chips.

Journal of the American Heart Association

Mathur T, Tronolone JJ, Jain A.
PMID: 34743553
J Am Heart Assoc. 2021 Nov 16;10(22):e022795. doi: 10.1161/JAHA.121.022795. Epub 2021 Nov 06.

Background Organ-on-chip technology has accelerated in vitro preclinical research of the vascular system, and a key strength of this platform is its promise to impact personalized medicine by providing a primary human cell-culture environment where endothelial cells are directly...

High-yield expression of periplasmic single-chain variable fragments by solid .

BioTechniques

Hanyu Y, Kato M.
PMID: 34841891
Biotechniques. 2022 Jan;72(1):29-32. doi: 10.2144/btn-2021-0093. Epub 2021 Nov 29.

High-yield expression of quality antibody fragments is indispensable for research and diagnosis. Most recombinant antibody fragments are expressed in

Inside the brain of a killer: the ethics of neuroimaging in a criminal conviction.

BioTechniques

Straiton J, Lake F.
PMID: 33567884
Biotechniques. 2021 Feb;70(2):69-71. doi: 10.2144/btn-2020-0171. Epub 2021 Feb 11.

With neuroimaging techniques being taken out of the lab and into the court, we ask whether brain scans can - or should - be used to explain a criminal act.

Showing 1 to 12 of 203 entries