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Nanomaterials (Basel). 2021 Dec 23;12(1). doi: 10.3390/nano12010028.

Green Synthesis of Gold Nanoparticles Using Upland Cress and Their Biochemical Characterization and Assessment.

Nanomaterials (Basel, Switzerland)

Noah Hutchinson, Yuelin Wu, Yale Wang, Muskan Kanungo, Anna DeBruine, Emma Kroll, De'Jorra Gilmore, Zachary Eckrose, Stephanie Gaston, Phoebe Matel, Matey Kaltchev, Anne-Marie Nickel, Subha Kumpaty, Xiaolin Hua, Wujie Zhang

Affiliations

  1. Department of Biomedical Engineering, Milwaukee School of Engineering, Milwaukee, WI 53202, USA.
  2. Department of Obstetrics, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 201204, China.
  3. Department of Mechanical Engineering, University of Milwaukee, Milwaukee, WI 53211, USA.
  4. Department of Physics and Chemistry, Milwaukee School of Engineering, Milwaukee, WI 53202, USA.
  5. Biomolecular Engineering Program, Milwaukee School of Engineering, Milwaukee, WI 53202, USA.
  6. Department of Mechanical Engineering, Milwaukee School of Engineering, Milwaukee, WI 53202, USA.

PMID: 35009978 PMCID: PMC8746345 DOI: 10.3390/nano12010028

Abstract

This research focuses on the plant-mediated green synthesis process to produce gold nanoparticles (Au NPs) using upland cress (Barbarea verna), as various biomolecules within the upland cress act as both reducing and capping agents. The synthesized gold nanoparticles were thoroughly characterized using UV-vis spectroscopy, surface charge (zeta potential) analysis, scanning electron microscopy-energy-dispersive X-ray spectroscopy (SEM-EDX), atomic force microscopy (AFM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), and X-ray diffraction (XRD). The results indicated the synthesized Au NPs are spherical and well-dispersed with an average diameter ~11 nm and a characteristic absorbance peak at ~529 nm. EDX results showed an 11.13% gold content. Colloidal Au NP stability was confirmed with a zeta potential (ζ) value of -36.8 mV. X-ray diffraction analysis verified the production of crystalline face-centered cubic gold. Moreover, the antimicrobial activity of the Au NPs was evaluated using Gram-negative

Keywords: catalysis; cytotoxicity; green synthesis; nanoparticles; upland cress

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