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Liu MH, Wang CR, Liu WJ, et al. Development of a single quantum dot-mediated FRET biosensor for amplification-free detection of ten-eleven translocation 2. Talanta. 2021;239:123135doi: 10.1016/j.talanta.2021.123135.
Liu, M. H., Wang, C. R., Liu, W. J., Xu, Q., & Zhang, C. Y. (2022). Development of a single quantum dot-mediated FRET biosensor for amplification-free detection of ten-eleven translocation 2. Talanta, 239123135. https://doi.org/10.1016/j.talanta.2021.123135
Liu, Ming-Hao, et al. "Development of a single quantum dot-mediated FRET biosensor for amplification-free detection of ten-eleven translocation 2." Talanta vol. 239 (2022): 123135. doi: https://doi.org/10.1016/j.talanta.2021.123135
Liu MH, Wang CR, Liu WJ, Xu Q, Zhang CY. Development of a single quantum dot-mediated FRET biosensor for amplification-free detection of ten-eleven translocation 2. Talanta. 2022 Mar 01;239:123135. doi: 10.1016/j.talanta.2021.123135. Epub 2021 Dec 09. PMID: 34920263.
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Talanta. 2022 Mar 01;239:123135. doi: 10.1016/j.talanta.2021.123135. Epub 2021 Dec 09.

Development of a single quantum dot-mediated FRET biosensor for amplification-free detection of ten-eleven translocation 2.

Talanta

Ming-Hao Liu, Chuan-Rui Wang, Wen-Jing Liu, Qinfeng Xu, Chun-Yang Zhang

Affiliations

  1. College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, 250014, China.
  2. School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, China.
  3. School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China. Electronic address: [email protected].
  4. College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, 250014, China. Electronic address: [email protected].

PMID: 34920263 DOI: 10.1016/j.talanta.2021.123135

Abstract

Ten-eleven translocation (TET) family proteins play key roles in multiple cellular processes by mediating the oxidation of 5-methylcytosine to directly participate in DNA demethylation, and often aberrantly expressed in various diseases. In this research, we develop a single-quantum-dot (QD)-mediated fluorescence resonance energy transfer (FRET) biosensor for amplification-free measurement of ten-eleven translocation 2 (TET2). When TET2 is present, it catalyzes the oxidation of 5-vinylcytosine in dsDNA to 5-formylmethylcytosine, and the subsequent labeling of dsDNA with Cy5 generates a biotinylated Cy5-dsDNA complex. Biotinylated Cy5-dsDNA complexes are conjugated to the streptavidin-coated 605QDs to obtain a Cy5-dsDNA-605QD nanostructures, inducing FRET from 605QD to Cy5. FRET signal can be simply measured by single-molecule counting. This biosensor enables homogeneous detection of TET2 with a limit of detection (LOD) of 0.042 ng/μL, and it can accurately measure cellular TET2 down to 1 cell. Moreover, this biosensor can be used to screen TET2 inhibitors, offering a new platform for TET2-related medical research and clinical diagnostics.

Copyright © 2021 Elsevier B.V. All rights reserved.

Keywords: Biosensor; Quantum dot; Single-molecule detection; Ten-eleven translocation

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