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Colsch B, Jackson SN, Dutta S, et al. Molecular Microscopy of Brain Gangliosides: Illustrating their Distribution in Hippocampal Cell Layers. ACS Chem Neurosci. 2011;2(4):213-222doi: 10.1021/cn100096h.
Colsch, B., Jackson, S. N., Dutta, S., & Woods, A. S. (2011). Molecular Microscopy of Brain Gangliosides: Illustrating their Distribution in Hippocampal Cell Layers. ACS chemical neuroscience, 2(4), 213-222. https://doi.org/10.1021/cn100096h
Colsch, Benoit, et al. "Molecular Microscopy of Brain Gangliosides: Illustrating their Distribution in Hippocampal Cell Layers." ACS chemical neuroscience vol. 2,4 (2011): 213-222. doi: https://doi.org/10.1021/cn100096h
Colsch B, Jackson SN, Dutta S, Woods AS. Molecular Microscopy of Brain Gangliosides: Illustrating their Distribution in Hippocampal Cell Layers. ACS Chem Neurosci. 2011 Feb 21;2(4):213-222. doi: 10.1021/cn100096h. PMID: 21961052; PMCID: PMC3181083.
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ACS Chem Neurosci. 2011 Feb 21;2(4):213-222. doi: 10.1021/cn100096h.

Molecular Microscopy of Brain Gangliosides: Illustrating their Distribution in Hippocampal Cell Layers.

ACS chemical neuroscience

Benoit Colsch, Shelley N Jackson, Sucharita Dutta, Amina S Woods

Affiliations

  1. Structural Biology Unit, Cellular Neurobiology Section, NIDA IRP, NIH, Baltimore, Maryland 21224, United States.

PMID: 21961052 PMCID: PMC3181083 DOI: 10.1021/cn100096h

Abstract

Gangliosides are amphiphilic molecules found in the outer layer of plasma membranes of all vertebrate cells. They play a major role in cell recognition and signaling and are involved in diseases affecting the central nervous system (CNS). We are reporting the differential distribution of ganglioside species in the rat brain's cerebrum, based on their ceramide associated core, and for the first time the presence of acetylation detected by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, which was used to map and image gangliosides with detailed structural information and histological accuracy. In the hippocampus, localization of the major species GM1, GD1, O-acetylGD1, GT1, and O-acetylGT1 depends on the sphingoïd base (d18:1 sphingosine or d20:1 eïcosasphingosine) in the molecular layer of the dentate gyrus (ML), which is made up of three distinct layers, the inner molecular layer (IML), which contains sphingosine exclusively, and the middle molecular layer (MML) and the outer molecular layer (OML) where eïcosasphingosine is the only sphingoïd base. These results demonstrate that there is a different distribution of gangliosides in neuronal axons and dendrites depending on the ceramide core of each layer. GM3, GM2, GD3, and GD2 contain sphingosine predominantly and are mainly present in body cell layers, which are made up of the pyramidal cell layer (Py) and the granular layer of the dentate gyrus (GL), in contrast with GQ1 and the O-acetylated forms of GD1, GT1, and GQ1 gangliosides, which contain both sphingoïd bases. However their distribution is based on the sialylated and acetylated oligosaccharide chains in the neuronal cell bodies.

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