Display options
Cite
Hayes CJ, Dowling CM, Dwane S, et al. Extracellular matrix gene expression profiling using microfluidics for colorectal carcinoma stratification. Biomicrofluidics. 2016;10(5):054124doi: 10.1063/1.4966245.
Hayes, C. J., Dowling, C. M., Dwane, S., McCumiskey, M. E., Tormey, S. M., Anne Merrigan, B., Coffey, J. C., Kiely, P. A., & Dalton, T. M. (2016). Extracellular matrix gene expression profiling using microfluidics for colorectal carcinoma stratification. Biomicrofluidics, 10(5), 054124. https://doi.org/10.1063/1.4966245
Hayes, Christopher J, et al. "Extracellular matrix gene expression profiling using microfluidics for colorectal carcinoma stratification." Biomicrofluidics vol. 10,5 (2016): 054124. doi: https://doi.org/10.1063/1.4966245
Hayes CJ, Dowling CM, Dwane S, McCumiskey ME, Tormey SM, Anne Merrigan B, Coffey JC, Kiely PA, Dalton TM. Extracellular matrix gene expression profiling using microfluidics for colorectal carcinoma stratification. Biomicrofluidics. 2016 Oct 31;10(5):054124. doi: 10.1063/1.4966245. eCollection 2016 Sep. PMID: 27822332; PMCID: PMC5097046.
Copy Download .nbib Format: NLM
Share it on

Biomicrofluidics. 2016 Oct 31;10(5):054124. doi: 10.1063/1.4966245. eCollection 2016 Sep.

Extracellular matrix gene expression profiling using microfluidics for colorectal carcinoma stratification.

Biomicrofluidics

Christopher J Hayes, Catriona M Dowling, Susan Dwane, Mary E McCumiskey, Shona M Tormey, B Anne Merrigan, John C Coffey, Patrick A Kiely, Tara M Dalton

Affiliations

  1. Stokes Laboratories, Bernal Institute, University of Limerick , Limerick, Ireland.
  2. Department of Surgery, University Hospital Limerick , Limerick, Ireland.

PMID: 27822332 PMCID: PMC5097046 DOI: 10.1063/1.4966245

Abstract

In cancer, biomarkers have many potential applications including generation of a differential diagnosis, prediction of response to treatment, and monitoring disease progression. Many molecular biomarkers have been put forward for different diseases but most of them do not possess the required specificity and sensitivity. A biomarker with a high sensitivity has a low specificity and vice versa. The inaccuracy of the biomarkers currently in use has led to a compelling need to identify more accurate markers with diagnostic and prognostic significance. The aim of the present study was to use a novel, droplet-based, microfluidic platform to evaluate the prognostic value of a panel of thirty-four genes that regulate the composition of extracellular matrices in colorectal carcinoma. Our method is a novel approach as it uses using continuous-flowing Polymerase Chain Reaction for the sensitive detection and accurate quantitation of gene expression. We identified a panel of relevant extracellular matrix genes whose expression levels were measured by real-time quantitative polymerase chain reaction using Taqman

References

  1. J Int Med Res. 2013 Aug;41(4):1228-35 - PubMed
  2. Oncol Rep. 2008 May;19(5):1285-91 - PubMed
  3. Clin Cancer Res. 2006 Dec 1;12(23):6998-7003 - PubMed
  4. Mol Cancer Res. 2006 May;4(5):293-302 - PubMed
  5. Cell Prolif. 2012 Oct;45(5):413-9 - PubMed
  6. Clin Cancer Res. 2011 Jun 15;17(12):4167-76 - PubMed
  7. World J Surg Oncol. 2014 Jan 29;12:24 - PubMed
  8. Br J Cancer. 2013 Aug 20;109(4):994-1003 - PubMed
  9. Clin Chem. 2009 Apr;55(4):611-22 - PubMed
  10. Curr Protoc Cell Biol. 2001 May;Chapter 10:Unit 10.1 - PubMed
  11. Genome Biol. 2002 Jun 18;3(7):RESEARCH0034 - PubMed
  12. F1000Res. 2016 Jan 22;5:99 - PubMed
  13. ChemMedChem. 2011 May 2;6(5):769-73 - PubMed
  14. J Cell Mol Med. 2012 Apr;16(4):683-90 - PubMed
  15. Oncogene. 2000 Nov 20;19(49):5574-81 - PubMed
  16. Gut. 1999 May;44(5):704-8 - PubMed
  17. PLoS One. 2013;8(3):e59918 - PubMed
  18. Mol Cell Biol. 2006 Jun;26(11):4041-51 - PubMed
  19. N Engl J Med. 2008 Oct 23;359(17):1757-65 - PubMed
  20. Curr Opin Cell Biol. 1993 Dec;5(6):1029-35 - PubMed
  21. Int J Cancer. 2015 Mar 1;136(5):E359-86 - PubMed
  22. Int J Oncol. 2006 Jul;29(1):83-94 - PubMed
  23. J Exp Clin Cancer Res. 2010 Jun 16;29:71 - PubMed
  24. J Clin Oncol. 2009 Nov 1;27(31):5131-7 - PubMed
  25. Oncogene. 2004 Feb 19;23(7):1377-91 - PubMed
  26. Eur Respir J. 2009 Aug;34(2):489-506 - PubMed
  27. Ann Oncol. 2005 Jan;16(1):102-8 - PubMed
  28. Oncology. 2013;84(2):81-91 - PubMed
  29. Cancer Lett. 2003 Jun 10;195(2):127-37 - PubMed
  30. Int J Mol Sci. 2015 May 27;16(6):12108-18 - PubMed
  31. PLoS One. 2012;7(8):e41001 - PubMed
  32. Lab Invest. 2015 Mar;95(3):308-19 - PubMed
  33. Br J Cancer. 2014 Jan 21;110(2):441-9 - PubMed
  34. Mol Pathol. 2002 Feb;55(1):34-9 - PubMed
  35. Asian J Surg. 2013 Jan;36(1):7-12 - PubMed
  36. Scand J Gastroenterol. 2000 Jul;35(7):747-52 - PubMed
  37. Anal Chem. 2003 Sep 15;75(18):4718-23 - PubMed
  38. Gut. 2005 Mar;54(3):374-84 - PubMed
  39. Anticancer Res. 2011 Jul;31(7):2541-5 - PubMed
  40. Cold Spring Harb Perspect Biol. 2011 Dec 01;3(12): - PubMed
  41. Cancer Res. 2015 Aug 15;75(16):3302-13 - PubMed
  42. Cancer. 2000 May 15;88(10):2201-9 - PubMed
  43. Tumour Biol. 2004 Jan-Apr;25(1-2):62-8 - PubMed
  44. Inflamm Bowel Dis. 2014 Jul;20(7):1147-56 - PubMed
  45. Oncoimmunology. 2012 Oct 1;1(7):1205-1207 - PubMed
  46. Curr Cancer Drug Targets. 2002 Sep;2(3):191-207 - PubMed
  47. Nat Rev Mol Cell Biol. 2014 Dec;15(12):786-801 - PubMed
  48. Ann Surg. 2014 Jul;260(1):94-102 - PubMed
  49. Surgery. 2005 Dec;138(6):1087-94 - PubMed
  50. Oncotarget. 2016 Apr 12;7(15):20919-33 - PubMed
  51. Cancers (Basel). 2014 Feb 10;6(1):366-75 - PubMed
  52. PLoS One. 2014 Sep 12;9(9):e106748 - PubMed
  53. J Gastroenterol. 2004;39(5):434-40 - PubMed
  54. Clin Cancer Res. 2007 Mar 1;13(5):1438-44 - PubMed
  55. Nat Rev Cancer. 2009 Apr;9(4):239-52 - PubMed
  56. PLoS One. 2014 Dec 30;9(12):e115276 - PubMed
  57. Proteomics. 2010 Mar;10(5):940-52 - PubMed
  58. J Nutr. 2001 Nov;131(11 Suppl):3109S-20S - PubMed
  59. Cancer Cell. 2004 Apr;5(4):329-39 - PubMed
  60. Crit Rev Oncol Hematol. 1995 Jul;19(3):183-232 - PubMed
  61. Oncol Rep. 2014 Jul;32(1):302-8 - PubMed
  62. Oncogene. 2005 Sep 8;24(40):6155-64 - PubMed
  63. In Vivo. 2014 Jan-Feb;28(1):105-10 - PubMed
  64. Int J Clin Exp Med. 2015 Apr 15;8(4):5883-9 - PubMed
  65. Biomol Detect Quantif. 2015 Jun 06;4:22-32 - PubMed
  66. Tech Coloproctol. 2004 Nov;8 Suppl 1:s123-5 - PubMed
  67. Gut. 1999 Aug;45(2):252-8 - PubMed
  68. Endocr Relat Cancer. 2005 Dec;12(4):891-901 - PubMed
  69. Nucleic Acids Res. 2001 May 1;29(9):e45 - PubMed
  70. Development. 2004 Dec;131(23):5883-95 - PubMed
  71. J Biol Chem. 2005 Mar 4;280(9):7624-33 - PubMed
  72. J Cancer Res Clin Oncol. 2012 Dec;138(12):2035-44 - PubMed
  73. Br J Cancer. 2003 Jul 7;89(1):158-67 - PubMed
  74. Nat Rev Cancer. 2014 Sep;14(9):598-610 - PubMed
  75. Clin Cancer Res. 2008 Sep 1;14(17):5555-64 - PubMed
  76. Int J Oncol. 2001 Feb;18(2):221-6 - PubMed
  77. Oncology. 2008;75(1-2):92-101 - PubMed
  78. Pathol Res Pract. 2014 Aug;210(8):473-6 - PubMed
  79. Int J Cancer. 2005 Mar 10;114(1):19-31 - PubMed
  80. Ann Ist Super Sanita. 2012;48(2):161-71 - PubMed
  81. Biotechnology (N Y). 1992 Apr;10(4):413-7 - PubMed
  82. Methods. 2001 Dec;25(4):402-8 - PubMed
  83. BMC Genomics. 2007 Aug 15;8:279 - PubMed
  84. Br J Cancer. 2012 May 22;106(11):1833-41 - PubMed
  85. Oncol Rep. 2008 Aug;20(2):359-64 - PubMed
  86. Endocr Relat Cancer. 2001 Mar;8(1):3-9 - PubMed
  87. Int J Colorectal Dis. 2014 May;29(5):563-9 - PubMed
  88. Int J Cancer. 2011 May 1;128(9):2038-49 - PubMed
  89. Cytokine Growth Factor Rev. 2001 Dec;12(4):375-91 - PubMed
  90. J Exp Clin Cancer Res. 2010 Nov 08;29:144 - PubMed
  91. J Natl Cancer Inst. 2000 Sep 20;92(18):1472-89 - PubMed
  92. Anticancer Agents Med Chem. 2013 May;13(4):584-94 - PubMed
  93. J Cell Biol. 2012 Feb 20;196(4):395-406 - PubMed
  94. BMC Mol Biol. 2010 Feb 01;11:12 - PubMed
  95. Clin Cancer Res. 1998 Jun;4(6):1507-10 - PubMed
  96. Clin Cancer Res. 2011 Dec 15;17(24):7654-63 - PubMed
  97. Oncol Rep. 2015 Jul;34(1):22-32 - PubMed
  98. Tumour Biol. 2013 Apr;34(2):735-41 - PubMed
  99. PLoS One. 2012;7(9):e46665 - PubMed
  100. Cancer Res. 2001 Mar 1;61(5):1919-26 - PubMed

Publication Types