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Showing 13 to 24 of 44 entries
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Devising Efficient Red-Shifting Strategies for Bioimaging: A Generalizable Donor-Acceptor Fluorophore Prototype.

Chemistry, an Asian journal

Chen C, Fang C.
PMID: 32216076
Chem Asian J. 2020 May 15;15(10):1514-1523. doi: 10.1002/asia.202000175. Epub 2020 Apr 08.

Long emission wavelengths, high fluorescence quantum yields (FQYs), and large Stokes shifts are highly desirable features for fluorescent probes in biological imaging. However, the current development of many fluorescent probes remains largely trial-and-error and lacks efficiency. Moreover, to achieve...

Distance Dependence of Single-Molecule Energy Transfer to Graphene Measured with DNA Origami Nanopositioners.

Nano letters

Kaminska I, Bohlen J, Rocchetti S, Selbach F, Acuna GP, Tinnefeld P.
PMID: 31251640
Nano Lett. 2019 Jul 10;19(7):4257-4262. doi: 10.1021/acs.nanolett.9b00172. Epub 2019 Jun 28.

Despite the thorough investigation of graphene since 2004, altering its surface chemistry and reproducible functionalization remain challenging. This hinders fabrication of more complex hybrid materials with controlled architectures, and as a consequence the development of sensitive and reliable sensors...

Analysis of protein changes using two-dimensional difference gel electrophoresis.

Methods in molecular biology (Clifton, N.J.)

Gao W.
PMID: 24623216
Methods Mol Biol. 2014;1105:17-30. doi: 10.1007/978-1-62703-739-6_2.

A protocol for protein analysis using two-dimensional difference gel electrophoresis (2D-DIGE) is described. 2D-DIGE is one of the most popular and versatile methods of protein separation among rapidly increasing proteomics technologies. Similar to traditional two-dimensional polyacrylamide gel electrophoresis (2D-PAGE),...

Fluorescence Guided Surgery.

Methods and applications in fluorescence

Stewart HL, Birch DJS.
PMID: 34399409
Methods Appl Fluoresc. 2021 Aug 26;9(4). doi: 10.1088/2050-6120/ac1dbb.

Fluorescence guided surgery (FGS) is an imaging technique that allows the surgeon to visualise different structures and types of tissue during a surgical procedure that may not be as visible under white light conditions. Due to the many potential...

Synthesis, crystals of centrosymmetric triphenylamine chromophores bearing prodigious two-photon absorption cross-section and biological imaging.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

Wang S, Xu S, Wang Y, Tian X, Zhang Y, Wang C, Wu J, Yang J, Tian Y.
PMID: 27816887
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Feb 15;173:871-879. doi: 10.1016/j.saa.2016.10.059. Epub 2016 Oct 31.

Two centrosymmetric D-π-D type triphenylamine chromophores with long π-conjugated bridge and strong electron-donating moiety were designed, synthesized and fully characterized. The crystal analysis revealed that multiple CH⋯π interactions existed in two chromophores, which played a crucial role in generating...

Quencher-free fluorescent method for homogeneously sensitive detection of microRNAs in human lung tissues.

Analytical chemistry

Zhu G, Liang L, Zhang CY.
PMID: 25356523
Anal Chem. 2014 Nov 18;86(22):11410-6. doi: 10.1021/ac503365z. Epub 2014 Nov 07.

Sensitive and accurate analysis of microRNA (miRNA) expression is imperative for understanding the biological functions of miRNAs and the early diagnosis of human cancers. Here, we develop a quencher-free fluorescent method for homogeneously sensitive detection of let-7a miRNA using...

Fluorescent kinase inhibitors as probes in cancer.

Chemical Society reviews

Usama SM, Zhao B, Burgess K.
PMID: 34291273
Chem Soc Rev. 2021 Sep 07;50(17):9794-9816. doi: 10.1039/d1cs00017a. Epub 2021 Jul 22.

Fluorescent dyes attached to kinase inhibitors (KIs) can be used to probe kinases in vitro, in cells, and in vivo. Ideal characteristics of the dyes vary with their intended applications. Fluorophores used in vitro may inform on kinase active...

The Promise of Aggregation-Induced Emission Luminogens for Detecting COVID-19.

Frontiers in immunology

Liu Z, Meng T, Tang X, Tian R, Guan W.
PMID: 33679789
Front Immunol. 2021 Feb 16;12:635558. doi: 10.3389/fimmu.2021.635558. eCollection 2021.

The long-term pandemic of coronavirus disease 2019 (COVID-19) requires sensitive and accurate diagnostic assays to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus and SARS-CoV-2 antibodies in infected individuals. Currently, RNA of SARS-CoV-2 virus is mainly detected by...

Analysis of cDNA microarray images.

Briefings in bioinformatics

Yang YH, Buckley MJ, Speed TP.
PMID: 11808746
Brief Bioinform. 2001 Dec;2(4):341-9. doi: 10.1093/bib/2.4.341.

Microarrays are part of a new class of biotechnologies that allow the monitoring of expression levels for thousands of genes simultaneously. Image analysis is an important aspect of microarray experiments, one that can have a potentially large impact on...

Fluorophore-assisted light inactivation for multiplex analysis of protein function in cellular processes.

Methods in enzymology

Eustace BK, Jay DG.
PMID: 12622173
Methods Enzymol. 2003;360:649-60. doi: 10.1016/s0076-6879(03)60133-3.

No abstract available.

Method for In-Vivo Fluorescence Imaging Contrast Enhancement through Light Modulation.

Journal of fluorescence

Kim J, Lee O, Ha S, Lee JW, Oh C.
PMID: 27633372
J Fluoresc. 2017 Jan;27(1):13-20. doi: 10.1007/s10895-016-1931-z. Epub 2016 Sep 16.

Early diagnosis is one of the most important factors that increase the therapeutic potential of the disease. Diagnoses conducted by conventional equipment are expensive, time-consuming, burdensome to patients, and do not have high success rates. Diagnostic methods have also...

Biosynthesized Gold Nanoclusters and Iron Complexes as Scaffolds for Multimodal Cancer Bioimaging.

Small (Weinheim an der Bergstrasse, Germany)

Zhao C, Du T, Rehman FU, Lai L, Liu X, Jiang X, Li X, Chen Y, Zhang H, Sun Y, Luo S, Jiang H, Selke M, Wang X.
PMID: 27672010
Small. 2016 Dec;12(45):6255-6265. doi: 10.1002/smll.201602526. Epub 2016 Sep 27.

Cancer treatment has a far greater chance of success if the neoplasm is diagnosed before the onset of metastasis to vital organs. Hence, cancer early diagnosis is extremely important and remains a major challenge in modern therapeutics. In this...

Showing 13 to 24 of 44 entries