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Showing 37 to 44 of 44 entries
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Nontrivial Effect of the Color-Exchange of a Donor/Acceptor Pair in the Engineering of Förster Resonance Energy Transfer (FRET)-Based Indicators.

ACS chemical biology

Ohta Y, Kamagata T, Mukai A, Takada S, Nagai T, Horikawa K.
PMID: 27232891
ACS Chem Biol. 2016 Jul 15;11(7):1816-22. doi: 10.1021/acschembio.6b00221. Epub 2016 Jun 01.

Genetically encoded indicators driven by the Förster resonance energy transfer (FRET) mechanism are reliable tools for live imaging. While the properties of FRET-based indicators have been improved over the years, they often suffer from a poor dynamic range due...

Rapid Sample Preparation and LAMP for Phytoplasma Detection.

Methods in molecular biology (Clifton, N.J.)

Hodgetts J.
PMID: 30362005
Methods Mol Biol. 2019;1875:187-201. doi: 10.1007/978-1-4939-8837-2_15.

Loop-mediated isothermal AMPlification (LAMP) allows the rapid detection of pathogens by polymerase-mediated amplification of target nucleic acid sequences at a single incubation temperature. LAMP can be combined with very simple sample preparation/crude DNA extraction protocols, allowing the method to...

Stoichiometry and Dispersity of DNA Nanostructures Using Photobleaching Pair-Correlation Analysis.

Bioconjugate chemistry

Hariri AA, Hamblin GD, Hardwick JS, Godin R, Desjardins JF, Wiseman PW, Sleiman HF, Cosa G.
PMID: 28777539
Bioconjug Chem. 2017 Sep 20;28(9):2340-2349. doi: 10.1021/acs.bioconjchem.7b00369. Epub 2017 Aug 22.

A wide variety of approaches have become available for the fabrication of nanomaterials with increasing degrees of complexity, precision, and speed while minimizing cost. Their quantitative characterization, however, remains a challenge. Analytical methods to better inspect and validate the...

Single-molecule confinement with uniform electrodynamic nanofluidics.

Lab on a chip

Ghosh S, Karedla N, Gregor I.
PMID: 32760965
Lab Chip. 2020 Sep 07;20(17):3249-3257. doi: 10.1039/d0lc00398k. Epub 2020 Aug 06.

To date, we could not engineer Nature's ability to dynamically handle diffusing single molecules in the liquid-phase as it takes place in pore-forming proteins and tunnelling nanotubes. Consistent handling of individual single molecules in a liquid is of paramount...

Novel live cell fluorescent probe for human-induced pluripotent stem cells highlights early reprogramming population.

Stem cell research & therapy

Sriram S, Kang NY, Subramanian S, Nandi T, Sudhagar S, Xing Q, Tong GJ, Chen AK, Srijaya TC, Tan P, Loh YH, Chang YT, Sugii S.
PMID: 33546754
Stem Cell Res Ther. 2021 Feb 05;12(1):113. doi: 10.1186/s13287-021-02171-6.

BACKGROUND: Despite recent rapid progress in method development and biological understanding of induced pluripotent stem (iPS) cells, there has been a relative shortage of tools that monitor the early reprogramming process into human iPS cells.METHODS: We screened the in-house...

Near-Infrared Thienoisoindigos with Aggregation-Induced Emission: Molecular Design, Optical Performance, and Bioimaging Application.

Analytical chemistry

Chen W, Zhang C, Chen H, Zang K, Liu SH, Xie Y, Tan Y, Yin J.
PMID: 33560116
Anal Chem. 2021 Feb 23;93(7):3378-3385. doi: 10.1021/acs.analchem.0c04260. Epub 2021 Feb 09.

Thienoisoindigo as a popular conjugated skeleton has been constantly applied in the construction of organic optoelectronic materials. Exploring its new applications in fluorescent sensors and bioimaging is helpful to extend its potential. In this work, a thienoisoindigo fluorophore was...

Insight into Fluorescence Imaging and Bioorthogonal Reactions in Biological Analysis.

Topics in current chemistry (Cham)

Ma Y, Chen Q, Pan X, Zhang J.
PMID: 33544237
Top Curr Chem (Cham). 2021 Feb 05;379(2):10. doi: 10.1007/s41061-020-00323-5.

Fluorescence imaging is an important method in the field of biomedicine. Fluorescence imaging is nondestructive, has high efficiency and sensitivity, high resolution and allows real-time dynamic monitoring of living cells. However, it also has some disadvantages, such as high...

An NIR-Fluorophore-Based Theranostic for Selective Initiation of Tumor Pyroptosis-Induced Immunotherapy.

Small (Weinheim an der Bergstrasse, Germany)

Wang K, Xiao X, Jiang M, Li J, Zhou J, Yuan Y.
PMID: 34323375
Small. 2021 Sep;17(36):e2102610. doi: 10.1002/smll.202102610. Epub 2021 Jul 29.

Pyroptosis is an inflammatory form of programmed cell death that can effectively eliminate malignant cells and boost anticancer immunity. However, most of the current pyroptosis inducers lack cell selectivity, which may cause severe side effects for cancer therapy. In...

Showing 37 to 44 of 44 entries