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Showing 1 to 12 of 31 entries
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Monosynaptic Interjoint Reflexes and their Central Modulation During Fictive Locomotion in Crayfish.

The European journal of neuroscience

El Manira A, DiCaprio RA, Cattaert D, Clarac F.
PMID: 12106221
Eur J Neurosci. 1991;3(12):1219-1231. doi: 10.1111/j.1460-9568.1991.tb00056.x.

An in vitro preparation of the crayfish nervous system has been utilized to study an interjoint reflex pathway and its variability during rhythmic locomotor activity. The coxo-basal chordotonal organ (CBCO) is a joint stretch receptor spanning the second joint...

New synthesis of (±)-cis-trikentrin A via tandem indole aryne cycloaddition/Negishi reaction. Applications to library development.

Tetrahedron letters

Brown N, Luo D, Decapo JA, Buszek KR.
PMID: 20877442
Tetrahedron Lett. 2009 Dec 23;50(51):7113-7115. doi: 10.1016/j.tetlet.2009.09.083.

We describe herein an efficient new route to the trikentrins and their related structures using a tandem 6,7-indolyne cycloaddition/Negishi cross-coupling reaction starting from a 4,6,7-tribromoindole (obtained in good yield via the Bartoli indole synthesis). The key step of this...

The Merkel Cell Polyomavirus T Antigens Function as Tumor Promoters in Murine Skin.

Cancers

Spurgeon ME, Liem A, Buehler D, Cheng J, DeCaprio JA, Lambert PF.
PMID: 33435392
Cancers (Basel). 2021 Jan 09;13(2). doi: 10.3390/cancers13020222.

Merkel cell polyomavirus (MCPyV) causes the majority of human Merkel cell carcinomas (MCC), a rare but highly aggressive form of skin cancer. We recently reported that constitutive expression of MCC tumor-derived MCPyV tumor (T) antigens in the skin of...

Simultaneous expression of MMB-FOXM1 complex components enables efficient bypass of senescence.

Scientific reports

Kumari R, Hummerich H, Shen X, Fischer M, Litovchick L, Mittnacht S, DeCaprio JA, Jat PS.
PMID: 34728711
Sci Rep. 2021 Nov 02;11(1):21506. doi: 10.1038/s41598-021-01012-z.

Cellular senescence is a stable cell cycle arrest that normal cells undergo after a finite number of divisions, in response to a variety of intrinsic and extrinsic stimuli. Although senescence is largely established and maintained by the p53/p21

Selecting the Antigen.

Cold Spring Harbor protocols

Greenfield EA, DeCaprio J, Brahmandam M.
PMID: 34853124
Cold Spring Harb Protoc. 2021 Dec 01;2021(12):pdb.top099945. doi: 10.1101/pdb.top099945.

The classical method for generating polyclonal or monoclonal antibodies relies on the in vivo humoral response of animals. Here we describe the factors that antigens can have that might influence the strength and quality of an antibody response. This...

Dynamic Properties of Electrotonic Coupling between Cells of Early Xenopus Embryos.

Biophysical journal

Dicaprio RA, French AS, Sanders EJ.
PMID: 19431351
Biophys J. 1974 May;14(5):387-411. doi: 10.1016/S0006-3495(74)85923-0.

Frequency response functions were measured between the cells of Xenopus laevis embryos during the first two cleavage stages. Linear systems theory was then used to produce electronic models which account for the electrical behavior of the systems. Coupling between...

Erratum: Detergent Lysis of Tissue Culture Cells for Immunoprecipitation.

Cold Spring Harbor protocols

DeCaprio J, Kohl TO.
PMID: 34074740
Cold Spring Harb Protoc. 2021 Jun 01;2021(6):pdb.err107714. doi: 10.1101/pdb.err107714.

No abstract available.

Momentary Associations of Osteoarthritis Pain and Affect: Depression as Moderator.

The journals of gerontology. Series B, Psychological sciences and social sciences

Parmelee PA, Behrens EA, Costlow Hill K, Cox BS, DeCaro JA, Keefe FJ, Smith DM.
PMID: 34865030
J Gerontol B Psychol Sci Soc Sci. 2021 Dec 05; doi: 10.1093/geronb/gbab221. Epub 2021 Dec 05.

OBJECTIVE: This research examined main and moderating effects of global depressive symptoms upon in-the-moment associations of pain and affect among individuals with knee osteoarthritis (OA). Effects of depression on short-term change in pain and affect were also examined.METHOD: Older...

Preparing mFc- and hFc-Fusion Proteins from Mammalian Cells.

Cold Spring Harbor protocols

Greenfield EA, DeCaprio J, Brahmandam M.
PMID: 34341175
Cold Spring Harb Protoc. 2021 Aug 02;2021(8). doi: 10.1101/pdb.prot100040.

Fc-fusion proteins are composed of an immunoglobulin Fc domain that is directly linked to the antigen of interest. Typically, these vectors will contain an amino-terminal signal sequence that permits trafficking to the cell surface and secretion into the media...

Bioinformatics-Guided Expansion and Discovery of Graspetides.

ACS chemical biology

Ramesh S, Guo X, DiCaprio AJ, De Lio AM, Harris LA, Kille BL, Pogorelov TV, Mitchell DA.
PMID: 34766760
ACS Chem Biol. 2021 Dec 17;16(12):2787-2797. doi: 10.1021/acschembio.1c00672. Epub 2021 Nov 12.

Graspetides are a class of ribosomally synthesized and post-translationally modified peptide natural products featuring ATP-grasp ligase-dependent formation of macrolactones/macrolactams. These modifications arise from serine, threonine, or lysine donor residues linked to aspartate or glutamate acceptor residues. Characterized graspetides include...

Predictors of immunotherapy benefit in Merkel cell carcinoma.

Oncotarget

Kacew AJ, Dharaneeswaran H, Starrett GJ, Thakuria M, LeBoeuf NR, Silk AW, DeCaprio JA, Hanna GJ.
PMID: 33315984
Oncotarget. 2020 Nov 24;11(47):4401-4410. doi: 10.18632/oncotarget.27823. eCollection 2020 Nov 24.

Merkel cell carcinoma is a rare cancer for which immune checkpoint blockade is standard-of-care for recurrent/metastatic disease. However, not all patients benefit from immunotherapy. A greater understanding of molecular mechanisms and predictive biomarkers are unmet needs. We retrospectively analyzed...

DNA Aptamer-Cyanine Complexes as Generic Colorimetric Small-Molecule Sensors.

Angewandte Chemie (International ed. in English)

Alkhamis O, Canoura J, Bukhryakov KV, Tarifa A, DeCaprio AP, Xiao Y.
PMID: 34706127
Angew Chem Int Ed Engl. 2021 Oct 27; doi: 10.1002/anie.202112305. Epub 2021 Oct 27.

Aptamers are promising biorecognition elements for sensors. However, aptamer-based assays often lack the requisite levels of sensitivity and/or selectivity because they typically employ structure-switching aptamers with attenuated affinity and/or utilize reporters that require aptamer labeling or which are susceptible...

Showing 1 to 12 of 31 entries